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Literature summary extracted from

  • Ishikawa, K.; Ishida, H.; Koyama, Y.; Kawarabayasi, Y.; Kawahara, J.; Matsui, E.; Matsui, I.
    Acylamino acid-releasing enzyme from the thermophilic archaeon Pyrococcus horikoshii (1998), J. Biol. Chem., 273, 17726-17731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.1 overexpression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.1 7.6
-
 N-acetyl-Ala-Ala
-
 Pyrococcus horikoshii
3.4.19.1 11
-
 N-acetyl-Leu p-nitroanilide
-
 Pyrococcus horikoshii
3.4.19.1 13
-
 N-acetyl-Ala-Ala-Ala-Ala
-
 Pyrococcus horikoshii
3.4.19.1 18.4
-
 N-acetyl-Ala p-nitroanilide
-
 Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.1 260000
-
 heat-activated enzyme, light-scattering photometry Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.1 Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.1 formylalanine-Ala-Ala + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 formylalanine-Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 formylmethionine-Ala + H2O
-
 Pyrococcus horikoshii formylmethionine + Ala
-
 ?
3.4.19.1 formylmethionine-Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 formylmethionine-Ala-Ser + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 formylmethionine-Leu-Gly + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 N-acetyl-Ala p-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-Ala + p-nitroaniline
-
 ?
3.4.19.1 N-acetyl-Ala-Ala + H2O
-
 Pyrococcus horikoshii N-acetyl-Ala + Ala
-
 ?
3.4.19.1 N-acetyl-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii N-acetyl-Ala + Ala-Ala-Ala
-
 ?
3.4.19.1 N-acetyl-Ala-Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 N-acetyl-Leu p-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-Leu + p-nitroaniline
-
 ?
3.4.19.1 N-acetyl-Met-Ala + H2O
-
 Pyrococcus horikoshii N-acetyl-Met + Ala
-
 ?
3.4.19.1 N-acetyl-Met-Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.4.19.1 N-acetyl-Tyr p-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-Tyr + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.1 AAREP
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.1 90
-
 at pH 5.4 Pyrococcus horikoshii
3.4.19.1 95
-
 heat-activated enzyme Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.1
-
 100 the major conformation of the enzyme remains stable Pyrococcus horikoshii
3.4.19.1 95
-
 24 h, stable Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.19.1 17.3
-
 N-acetyl-Ala p-nitroanilide
-
 Pyrococcus horikoshii
3.4.19.1 281
-
 N-acetyl-Ala-Ala-Ala-Ala
-
 Pyrococcus horikoshii
3.4.19.1 330
-
 N-acetyl-Leu p-nitroanilide
-
 Pyrococcus horikoshii
3.4.19.1 790
-
 N-acetyl-Ala-Ala
-
 Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.1 4.8 5.5 at 85°C Pyrococcus horikoshii
3.4.19.1 7
-
 heat activated enzyme Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.19.1 5 8 about 60% of maximal activity at pH 5 and pH 8 Pyrococcus horikoshii