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Literature summary extracted from

  • Farries, T.C.; Harris, A.; Auffret, A.D.; Aitken, A.
    Removal of N-acetyl groups from blocked peptides with acylpeptide hydrolase (1991), Eur. J. Biochem., 196, 679-685.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.1 analysis the enzyme is a tool in sequencing blocked peptides and proteins. Useful for unblocking acetylated proteins prior to protein sequence analysis Ovis aries

General Stability

EC Number General Stability Organism
3.4.19.1 coupling to CNBr-Sepharose and cross-linking with dimethylsuberimidate improves the thermal stability. This treatment also enhances resistance to inactivation by ionic detergent and the organic solvent N,N-dimethylformamide Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.1 activated-thiol-Sepharose
-
 Ovis aries
3.4.19.1 bis(sulfosuccinimidyl)suberate
-
 Ovis aries
3.4.19.1 carbodiimide/aminoalkyl-agarose
-
 Ovis aries
3.4.19.1 carbodiimide/dicarboxylic acid
-
 Ovis aries
3.4.19.1 epoxy-Sepharose
-
 Ovis aries
3.4.19.1 Glutaraldehyde
-
 Ovis aries
3.4.19.1 N,N-Dimethylformamide
-
 Ovis aries
3.4.19.1 N-hydroxysuccinimide agarose
-
 Ovis aries
3.4.19.1 SDS
-
 Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.1 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.1
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.1 liver
-
 Ovis aries
-

Synonyms

EC Number Synonyms Comment Organism
3.4.19.1 APH
-
 Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.1 additional information
-
 coupling to CNBr-Sepharose and cross-linking with dimethylsuberimidate improve the thermal stability Ovis aries