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Literature summary extracted from

  • Huang, K.; Takagaki, M.; Kani, K.; Ohkubo, I.
    Dipeptidyl peptidase II from porcine seminal plasma: purification, characterization, and its homology to granzymes, cytosolic cell proteinases (CCP 1-4) (1996), Biochim. Biophys. Acta, 1290, 149-156.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.14.2 resistant against several repeated cycles of freezing and thawing Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.2 4-(2-aminoethyl)benzenesulfonyl fluoride
-
 Sus scrofa
3.4.14.2 Cu2+
-
 Sus scrofa
3.4.14.2 diisopropyl fluorophosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.2 0.36
-
 L-Lys-L-Ala-7-amido-4-methylcoumarin
-
 Sus scrofa
3.4.14.2 1.33
-
 Gly-Pro-4-methylcoumarin 7-amide
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.2 58000
-
 3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
3.4.14.2 61000
-
 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa
3.4.14.2 185000
-
 gel filtration Sus scrofa
3.4.14.2 200000
-
 non-denaturing PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.2 seminal plasma
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.2 0.803
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.4.14.2 -30°C, stable for at least several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.2 Gly-Pro-2-(4-methoxy)-naphthylamide + H2O
-
 Sus scrofa ?
-
 ?
3.4.14.2 Gly-Pro-4-methylcoumarin 7-amide + H2O
-
 Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.2 L-Lys-L-Ala-7-amido-4-methylcoumarin + H2O
-
 Sus scrofa L-Lys-L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.14.2 Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O
-
 Sus scrofa ?
-
 ?
3.4.14.2 Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
 Sus scrofa Lys-Ala + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.2 trimer 3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
3.4.14.2 trimer 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.2 55
-
 30 min, stable up to Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.2 6
-
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.2 3.5 10 4°C, 24 h, stable Sus scrofa