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Literature summary extracted from

  • Mentlein, R.; Struckhoff, G.
    Purification of two dipeptidyl aminopeptidases II from rat brain and their action on proline-containing neuropeptides (1989), J. Neurochem., 52, 1284-1293.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.2 bestatin 1 mM, 12% inhibition of DPP II-S, 21% inhibition of DPP II-M; no inhibition Rattus norvegicus
3.4.14.2 diisopropyl fluorophosphate
-
 Rattus norvegicus
3.4.14.2 additional information no inhibition by bacitracin Rattus norvegicus
3.4.14.2 N6-benzyloxycarbonyl-Lys-Pro 1 mM, 31% inhibition of DPP II-S, 41% inhibition of DPP II-M Rattus norvegicus
3.4.14.2 Na+ 400 mM NaCl, 56% inhibition of DPP II-M, 31% inhibition of DPP II-S Rattus norvegicus
3.4.14.2 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.4.14.2 phenylmethylsulfonyl fluoride
-
 Rattus norvegicus
3.4.14.2 Tris
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.2 0.018
-
 Gly-Pro-4-nitroanilide enzyme form DPP II-S Rattus norvegicus
3.4.14.2 0.032
-
 Gly-Pro-4-nitroanilide enzyme form DPP II-M Rattus norvegicus
3.4.14.2 0.068
-
 Arg-Pro-4-nitroanilide enzyme form DPP II-M Rattus norvegicus
3.4.14.2 0.087
-
 Lys-Ala-2-naphthylamide enzyme form DPP II-S Rattus norvegicus
3.4.14.2 0.21
-
 Lys-Ala-2-naphthylamide enzyme form DPP II-M Rattus norvegicus
3.4.14.2 0.502
-
 His-Pro-Val enzyme form DPP II-S Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.2 membrane enzyme form DPP II-S Rattus norvegicus 16020
-
3.4.14.2 soluble enzyme form DPP II-M Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.2 52500
-
 2 * 52500, SDS-PAGE Rattus norvegicus
3.4.14.2 110000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.2 Rattus norvegicus
-
 two enzyme forms: DPP II-M and DPP II-S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.2 enzyme form DPP II-S and DPP II-M Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.2 brain
-
 Rattus norvegicus
-
3.4.14.2 hypophysis
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.4.14.2 -20°C, pH 6.0, 20 mM BisTris-HCl buffer, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.2 Ala-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Ala-Ala + 2-naphthylamine
-
 ?
3.4.14.2 Arg-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.2 bradykinin fragment 1-3 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.2 bradykinin fragment 1-5 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.2 Gly-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.2 His-Pro-Val + H2O
-
 Rattus norvegicus His-Pro + Val
-
 ?
3.4.14.2 Lys-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Lys-Ala + 2-naphthylamine
-
 ?
3.4.14.2 additional information no activity with bradykinin Rattus norvegicus ?
-
 ?
3.4.14.2 Phe-Pro-2-naphthylamide + H2O
-
 Rattus norvegicus Phe-Pro + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.2 dimer 2 * 52500, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.14.2 DPP II
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.2 5.5
-
 hydrolysis of Gly-Pro-4-nitroanilide or Arg-Pro-4-nitroanilide Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.2 3
-
 4°C, 24 h, loss of activity of DPP II-M Rattus norvegicus
3.4.14.2 4 8 4°C, 24 h, DPP II-M is stable Rattus norvegicus