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Literature summary extracted from

  • Larson, T.J.; van Loo-Bhattacharya, A.T.
    Purification and characterization of glpQ-encoded glycerophosphodiester phosphodiesterase from Escherichia coli K-12 (1988), Arch. Biochem. Biophys., 260, 577-584.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.2 EDTA complete inhibition at 1 mM Escherichia coli
3.1.4.2 EGTA complete inhibition at 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.2 0.24
-
glycerophosphoethanolamine
-
Escherichia coli
3.1.4.2 0.28
-
glycerophosphocholine addition of Ca2+ decreases Km and increases Vmax. Escherichia coli
3.1.4.2 0.35
-
glycerophosphoglycerol
-
Escherichia coli
3.1.4.2 0.59
-
glycerophosphoserine
-
Escherichia coli
3.1.4.2 1
-
glycerophosphoinositol
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Ca2+ fully restored activity if chelating agents used Escherichia coli
3.1.4.2 Cd2+ fully restored activity if chelating agents used Escherichia coli
3.1.4.2 Cu2+ partially restored activity if chelating agents used Escherichia coli
3.1.4.2 Mn2+ partially restored activity if chelating agents used Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.2 40000
-
SDS-PAGE analysis Escherichia coli
3.1.4.2 70000
-
gel filtration chromatography Escherichia coli
3.1.4.2 75000
-
native PAGE analysis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2 to homogenity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.2 325
-
90% homogenity Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.4.2 -75°C, 25 mM Tris-HCl pH 7.4, 0.1 mM DTT, 10% glycerol, several months Escherichia coli
3.1.4.2 room temperature, 0.02 M Tris-HCl pH 7.5, 24 h Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 glycerophosphoethanolamine + H2O
-
Escherichia coli ?
-
?
3.1.4.2 glycerophosphoglycerol + H2O
-
Escherichia coli glycerol + glycerol 3-phosphate
-
?
3.1.4.2 glycerophosphoinositol + H2O
-
Escherichia coli inositol + glycerol 3-phosphate
-
?
3.1.4.2 glycerophosphoserine + H2O
-
Escherichia coli serine + glycerol 3-phosphate
-
?
3.1.4.2 sn-glycero 3-phosphorylcholine + H2O
-
Escherichia coli sn-glycerol 3-phosphate + choline
-
?
3.1.4.2 sn-glycero 3-phosphorylethanolamine + H2O
-
Escherichia coli sn-glycerol 3-phosphate + ethanolamine
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.2 dimer alpha2, 2 * 37500-40000, native PAGE and SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 glycerophosphodiester phosphodiesterase
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.2 220
-
sn-glycero 3-phosphorylcholine
-
Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.2 7.8
-
using two-step assay Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.2 6.7 9 less than 50% activity below pH6 6.7 and above pH 9.0 Escherichia coli