EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.2 | expression in Escherichia coli | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.2 | EDTA | complete inhibition at 1 mM | Escherichia coli | |
3.1.4.2 | EGTA | complete inhibition at 1 mM | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.2 | 0.24 | - |
glycerophosphoethanolamine | - |
Escherichia coli | |
3.1.4.2 | 0.28 | - |
glycerophosphocholine | addition of Ca2+ decreases Km and increases Vmax. | Escherichia coli | |
3.1.4.2 | 0.35 | - |
glycerophosphoglycerol | - |
Escherichia coli | |
3.1.4.2 | 0.59 | - |
glycerophosphoserine | - |
Escherichia coli | |
3.1.4.2 | 1 | - |
glycerophosphoinositol | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.2 | Ca2+ | fully restored activity if chelating agents used | Escherichia coli | |
3.1.4.2 | Cd2+ | fully restored activity if chelating agents used | Escherichia coli | |
3.1.4.2 | Cu2+ | partially restored activity if chelating agents used | Escherichia coli | |
3.1.4.2 | Mn2+ | partially restored activity if chelating agents used | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.2 | 40000 | - |
SDS-PAGE analysis | Escherichia coli |
3.1.4.2 | 70000 | - |
gel filtration chromatography | Escherichia coli |
3.1.4.2 | 75000 | - |
native PAGE analysis | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.2 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.2 | to homogenity | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.2 | 325 | - |
90% homogenity | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.4.2 | -75°C, 25 mM Tris-HCl pH 7.4, 0.1 mM DTT, 10% glycerol, several months | Escherichia coli |
3.1.4.2 | room temperature, 0.02 M Tris-HCl pH 7.5, 24 h | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.2 | glycerophosphoethanolamine + H2O | - |
Escherichia coli | ? | - |
? | |
3.1.4.2 | glycerophosphoglycerol + H2O | - |
Escherichia coli | glycerol + glycerol 3-phosphate | - |
? | |
3.1.4.2 | glycerophosphoinositol + H2O | - |
Escherichia coli | inositol + glycerol 3-phosphate | - |
? | |
3.1.4.2 | glycerophosphoserine + H2O | - |
Escherichia coli | serine + glycerol 3-phosphate | - |
? | |
3.1.4.2 | sn-glycero 3-phosphorylcholine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + choline | - |
? | |
3.1.4.2 | sn-glycero 3-phosphorylethanolamine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + ethanolamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.2 | dimer | alpha2, 2 * 37500-40000, native PAGE and SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.2 | glycerophosphodiester phosphodiesterase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.2 | 220 | - |
sn-glycero 3-phosphorylcholine | - |
Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.2 | 7.8 | - |
using two-step assay | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.2 | 6.7 | 9 | less than 50% activity below pH6 6.7 and above pH 9.0 | Escherichia coli |