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Literature summary extracted from

  • Kazama, H.; Tabat, N.; Ohgi, K.; Irie, M.
    Purification and chacterization of a nuclease (3'-nucleotidase) from Penicillium sp. (1990), Chem. Pharm. Bull., 38, 3081-3085.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.6 Zn2+ 2 mol Zn2+ per mol of enzyme, nuclease PA3, the Zn atoms are chelating to some of the His residues and have important roles in the enzymatic activity Penicillium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.6 32000
-
 x * 32000, nuclease PA3, SDS-PAGE Penicillium sp.
3.1.3.6 33000
-
 x * 33000, nuclease PA2, SDS-PAGE Penicillium sp.
3.1.3.6 35000
-
 x * 35000, nuclease PA1, SDS-PAGE Penicillium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Penicillium sp.
-
 nuclease PA1, nuclease PA2, nuclease PA3
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.6 side-chain modification glycoprotein Penicillium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.6 nuclease PA1, nuclease PA2, nuclease PA3 Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Penicillium sp. adenosine + phosphate
-
 ?
3.1.3.6 3'-CMP + H2O
-
 Penicillium sp. cytosine + phosphate
-
 ?
3.1.3.6 3'-GMP + H2O
-
 Penicillium sp. guanosine + phosphate
-
 ?
3.1.3.6 3'-UMP + H2O
-
 Penicillium sp. uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.6 ? x * 32000, nuclease PA3, SDS-PAGE Penicillium sp.
3.1.3.6 ? x * 35000, nuclease PA1, SDS-PAGE Penicillium sp.
3.1.3.6 ? x * 33000, nuclease PA2, SDS-PAGE Penicillium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 6.5
-
 substrates 3'-CMP and 3'-UMP Penicillium sp.
3.1.3.6 7
-
 substrate 3'-AMP Penicillium sp.
3.1.3.6 8
-
 substrate 3'-GMP Penicillium sp.