BRENDA - Enzyme Database show

A 5'-nucleotidase from Neurospora crassa

Olson, A.C.; Fraser, M.J.; Biochim. Biophys. Acta 334, 156-167 (1974)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
glycine
activation
Neurospora crassa
3.1.3.5
histidine
activation
Neurospora crassa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ATP
-
Neurospora crassa
3.1.3.5
deoxyadenosine
-
Neurospora crassa
3.1.3.5
deoxycytidine
-
Neurospora crassa
3.1.3.5
KCl
-
Neurospora crassa
3.1.3.5
thymidine
-
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.015
-
5'-dTMP
-
Neurospora crassa
3.1.3.5
0.083
-
5'-dGMP
-
Neurospora crassa
3.1.3.5
0.125
-
5'-dAMP
-
Neurospora crassa
3.1.3.5
0.13
-
5'-AMP
-
Neurospora crassa
3.1.3.5
0.15
-
5'-dCMP
-
Neurospora crassa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
NH4Cl
activation
Neurospora crassa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Neurospora crassa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
copurification with single-strand-specific exonuclease
Neurospora crassa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
conidium
-
Neurospora crassa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95051
Neurospora crassa
adenosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-CMP + H2O
-
95051
Neurospora crassa
cytidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95051
Neurospora crassa
deoxyadenosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95051
Neurospora crassa
deoxycytidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95051
Neurospora crassa
deoxyguanosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
95051
Neurospora crassa
deoxythymidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-GMP + H2O
-
95051
Neurospora crassa
guanosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-IMP + H2O
-
95051
Neurospora crassa
inosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-UMP + H2O
-
95051
Neurospora crassa
uridine + phosphate
-
95051
Neurospora crassa
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
5.5
-
second optimum at pH 8.3
Neurospora crassa
3.1.3.5
8.3
-
second optimum at pH 5.5
Neurospora crassa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
glycine
activation
Neurospora crassa
3.1.3.5
histidine
activation
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ATP
-
Neurospora crassa
3.1.3.5
deoxyadenosine
-
Neurospora crassa
3.1.3.5
deoxycytidine
-
Neurospora crassa
3.1.3.5
KCl
-
Neurospora crassa
3.1.3.5
thymidine
-
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.015
-
5'-dTMP
-
Neurospora crassa
3.1.3.5
0.083
-
5'-dGMP
-
Neurospora crassa
3.1.3.5
0.125
-
5'-dAMP
-
Neurospora crassa
3.1.3.5
0.13
-
5'-AMP
-
Neurospora crassa
3.1.3.5
0.15
-
5'-dCMP
-
Neurospora crassa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
NH4Cl
activation
Neurospora crassa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
copurification with single-strand-specific exonuclease
Neurospora crassa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
conidium
-
Neurospora crassa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95051
Neurospora crassa
adenosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-CMP + H2O
-
95051
Neurospora crassa
cytidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95051
Neurospora crassa
deoxyadenosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95051
Neurospora crassa
deoxycytidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95051
Neurospora crassa
deoxyguanosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
95051
Neurospora crassa
deoxythymidine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-GMP + H2O
-
95051
Neurospora crassa
guanosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-IMP + H2O
-
95051
Neurospora crassa
inosine + phosphate
-
95051
Neurospora crassa
?
3.1.3.5
5'-UMP + H2O
-
95051
Neurospora crassa
uridine + phosphate
-
95051
Neurospora crassa
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
5.5
-
second optimum at pH 8.3
Neurospora crassa
3.1.3.5
8.3
-
second optimum at pH 5.5
Neurospora crassa