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Literature summary extracted from

  • Widnell, C.C.
    Purification of rat liver 5'-nucleotidase as a complex with sphingomyelin (1974), Methods Enzymol., 32, 368-374.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 adenosine
-
 Rattus norvegicus
3.1.3.5 Co2+ 0.1 mM activate, 4 mM inhibit, plasma membrane enzyme Rattus norvegicus
3.1.3.5 EDTA
-
 Rattus norvegicus
3.1.3.5 Fe2+ plasma membrane enzyme Rattus norvegicus
3.1.3.5 Mn2+ 0.1 mM activate, 4 mM inhibit, plasma membrane enzyme Rattus norvegicus
3.1.3.5 Ni2+ plasma membrane enzyme Rattus norvegicus
3.1.3.5 Pb2+ plasma membrane enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 Golgi apparatus
-
 Rattus norvegicus 5794
-
3.1.3.5 microsome
-
 Rattus norvegicus
-
-
3.1.3.5 mitochondrial membrane
-
 Rattus norvegicus 31966
-
3.1.3.5 plasma membrane
-
 Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Co2+ 0.1 mM activates, 4 mM inhibits, plasma membrane enzyme Rattus norvegicus
3.1.3.5 Mg2+ activation, membrane-bound enzyme Rattus norvegicus
3.1.3.5 Mn2+ 0.1 mM activates, 4 mM inhibits, plasma membrane enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 liver, membrane-bound Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 liver membrane-bound Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 124
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 0°C, 0.1 M Tris-HCl, pH 7.5, 1 mM MgCl2, 1 mM 5'-AMP, stable for 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Rattus norvegicus uridine + phosphate
-
 ?