BRENDA - Enzyme Database show

5'-Nucleotidase from rat heart

Naito, Y.; Lowenstein, J.M.; Biochemistry 20, 5188-5194 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ADP
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.002
-
adenosine 5'-O-thiophosphate
-
Rattus norvegicus
3.1.3.5
0.0053
-
6-chloropurine ribonucleoside 5'-monophosphate
-
Rattus norvegicus
3.1.3.5
0.0056
-
5'-AMP
-
Rattus norvegicus
3.1.3.5
0.0064
-
5'-GMP
-
Rattus norvegicus
3.1.3.5
0.0065
-
1,N6-ethenoadenosine 5'-phosphate
-
Rattus norvegicus
3.1.3.5
0.007
-
3-beta-ribofuranosyladenine 5'-monophosphate
-
Rattus norvegicus
3.1.3.5
0.008
-
5'-IMP
-
Rattus norvegicus
3.1.3.5
0.014
-
5'-dTMP
-
Rattus norvegicus
3.1.3.5
0.014
-
5'-UMP
-
Rattus norvegicus
3.1.3.5
0.035
-
5'-CMP
-
Rattus norvegicus
3.1.3.5
0.077
-
5'-dAMP
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation, membrane-bound enzyme
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
74000
-
2 * 74000, heart, membrane-bound, SDS-PAGE
Rattus norvegicus
3.1.3.5
147000
-
heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Rattus norvegicus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
side-chain modification
glycoprotein
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
heart, membrane-bound
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.5
4°C, 50 mM Tris-HCl, pH 7.5, 50 mM sodium cholate, 1 M NaCl
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
1,N6-ethenoadenosine 5'-phosphate + H2O
45% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
1,N6-ethenoadenosine ribonucleoside + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
3-beta-ribofuranosyladenine 5'-monophosphate + H2O
24% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
3-beta-ribofuranosyladenine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-AMP + H2O
-
95042
Rattus norvegicus
adenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-CMP + H2O
87% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
cytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95042
Rattus norvegicus
deoxyadenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dAMP + H2O
20% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxyadenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dCMP + H2O
28% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxycytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95042
Rattus norvegicus
deoxycytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
95042
Rattus norvegicus
deoxythymidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dTMP + H2O
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxythymidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-GMP + H2O
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
guanosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-IMP + H2O
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
93% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
uridine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
6-chloropurine ribonucleoside 5'-monophosphate + H2O
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
6-chloropurine ribonucleoside + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
adenosine 5'-O-thiophosphate + H2O
6% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
dimer
2 * 74000, heart, membrane-bound, SDS-PAGE
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ADP
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.002
-
adenosine 5'-O-thiophosphate
-
Rattus norvegicus
3.1.3.5
0.0053
-
6-chloropurine ribonucleoside 5'-monophosphate
-
Rattus norvegicus
3.1.3.5
0.0056
-
5'-AMP
-
Rattus norvegicus
3.1.3.5
0.0064
-
5'-GMP
-
Rattus norvegicus
3.1.3.5
0.0065
-
1,N6-ethenoadenosine 5'-phosphate
-
Rattus norvegicus
3.1.3.5
0.007
-
3-beta-ribofuranosyladenine 5'-monophosphate
-
Rattus norvegicus
3.1.3.5
0.008
-
5'-IMP
-
Rattus norvegicus
3.1.3.5
0.014
-
5'-dTMP
-
Rattus norvegicus
3.1.3.5
0.014
-
5'-UMP
-
Rattus norvegicus
3.1.3.5
0.035
-
5'-CMP
-
Rattus norvegicus
3.1.3.5
0.077
-
5'-dAMP
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation, membrane-bound enzyme
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
74000
-
2 * 74000, heart, membrane-bound, SDS-PAGE
Rattus norvegicus
3.1.3.5
147000
-
heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate
Rattus norvegicus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
side-chain modification
glycoprotein
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
heart, membrane-bound
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.5
4°C, 50 mM Tris-HCl, pH 7.5, 50 mM sodium cholate, 1 M NaCl
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
1,N6-ethenoadenosine 5'-phosphate + H2O
45% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
1,N6-ethenoadenosine ribonucleoside + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
3-beta-ribofuranosyladenine 5'-monophosphate + H2O
24% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
3-beta-ribofuranosyladenine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-AMP + H2O
-
95042
Rattus norvegicus
adenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-CMP + H2O
87% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
cytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95042
Rattus norvegicus
deoxyadenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dAMP + H2O
20% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxyadenosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dCMP + H2O
28% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxycytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95042
Rattus norvegicus
deoxycytidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
95042
Rattus norvegicus
deoxythymidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-dTMP + H2O
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
deoxythymidine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-GMP + H2O
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
guanosine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
5'-IMP + H2O
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
93% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
uridine + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
6-chloropurine ribonucleoside 5'-monophosphate + H2O
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
6-chloropurine ribonucleoside + phosphate
-
95042
Rattus norvegicus
?
3.1.3.5
adenosine 5'-O-thiophosphate + H2O
6% of the activity with 5'-AMP, heart, membrane-bound enzyme
95042
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
dimer
2 * 74000, heart, membrane-bound, SDS-PAGE
Rattus norvegicus