BRENDA - Enzyme Database show

Human placental cytoplasmic 5'-nucleotidase. Kinetic properties and inhibition

Madrid-Marina, V.; Fox, I.H.; J. Biol. Chem. 261, 444-452 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
adenosine 5'-(alpha,beta-methylene)diphosphate
-
Homo sapiens
3.1.3.5
adenosine 5'-(alpha,beta-methylene)triphosphate
-
Homo sapiens
3.1.3.5
ADP
-
Homo sapiens
3.1.3.5
ATP
-
Homo sapiens
3.1.3.5
CDP
-
Homo sapiens
3.1.3.5
CTP
-
Homo sapiens
3.1.3.5
dADP
-
Homo sapiens
3.1.3.5
dATP
-
Homo sapiens
3.1.3.5
dCTP
-
Homo sapiens
3.1.3.5
dGDP
-
Homo sapiens
3.1.3.5
dGTP
-
Homo sapiens
3.1.3.5
dITP
-
Homo sapiens
3.1.3.5
dUTP
-
Homo sapiens
3.1.3.5
GDP
-
Homo sapiens
3.1.3.5
GTP
-
Homo sapiens
3.1.3.5
IDP
-
Homo sapiens
3.1.3.5
ITP
-
Homo sapiens
3.1.3.5
NAD+
-
Homo sapiens
3.1.3.5
NADH
-
Homo sapiens
3.1.3.5
NADP+
-
Homo sapiens
3.1.3.5
NADPH
-
Homo sapiens
3.1.3.5
TDP
-
Homo sapiens
3.1.3.5
TTP
-
Homo sapiens
3.1.3.5
UDP
-
Homo sapiens
3.1.3.5
UTP
-
Homo sapiens
3.1.3.5
XTP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.002
-
5'-CMP
-
Homo sapiens
3.1.3.5
0.014
-
5'-UMP
-
Homo sapiens
3.1.3.5
0.017
-
5'-dUMP
-
Homo sapiens
3.1.3.5
0.017
-
5'-GMP
-
Homo sapiens
3.1.3.5
0.018
-
5'-AMP
-
Homo sapiens
3.1.3.5
0.023
-
5'-dCMP
-
Homo sapiens
3.1.3.5
0.03
-
5'-IMP
-
Homo sapiens
3.1.3.5
0.035
-
5'-dAMP
-
Homo sapiens
3.1.3.5
0.044
-
5'-dGMP
-
Homo sapiens
3.1.3.5
0.057
-
5'-dIMP
-
Homo sapiens
3.1.3.5
0.065
-
5'-XMP
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytoplasm
two types of cytoplasmic enzymes: one type characterized by millimolar Km values, activation by ATP and ADP, regulation by phosphate. A second type characterized by micromolar Km values, inhibition by ATP, ADP and phosphate, the latter at high concentration
Homo sapiens
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95035
Homo sapiens
adenosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
-
95035
Homo sapiens
cytidine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95035
Homo sapiens
deoxyadenosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95035
Homo sapiens
deoxycytidine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95035
Homo sapiens
deoxyguanosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
95035
Homo sapiens
deoxyinosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
95035
Homo sapiens
deoxyuridne + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
-
95035
Homo sapiens
guanosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-IMP + H2O
-
95035
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
-
95035
Homo sapiens
uridine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-XMP + H2O
-
95035
Homo sapiens
xanthosine + phosphate
-
95035
Homo sapiens
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
adenosine 5'-(alpha,beta-methylene)diphosphate
-
Homo sapiens
3.1.3.5
adenosine 5'-(alpha,beta-methylene)triphosphate
-
Homo sapiens
3.1.3.5
ADP
-
Homo sapiens
3.1.3.5
ATP
-
Homo sapiens
3.1.3.5
CDP
-
Homo sapiens
3.1.3.5
CTP
-
Homo sapiens
3.1.3.5
dADP
-
Homo sapiens
3.1.3.5
dATP
-
Homo sapiens
3.1.3.5
dCTP
-
Homo sapiens
3.1.3.5
dGDP
-
Homo sapiens
3.1.3.5
dGTP
-
Homo sapiens
3.1.3.5
dITP
-
Homo sapiens
3.1.3.5
dUTP
-
Homo sapiens
3.1.3.5
GDP
-
Homo sapiens
3.1.3.5
GTP
-
Homo sapiens
3.1.3.5
IDP
-
Homo sapiens
3.1.3.5
ITP
-
Homo sapiens
3.1.3.5
NAD+
-
Homo sapiens
3.1.3.5
NADH
-
Homo sapiens
3.1.3.5
NADP+
-
Homo sapiens
3.1.3.5
NADPH
-
Homo sapiens
3.1.3.5
TDP
-
Homo sapiens
3.1.3.5
TTP
-
Homo sapiens
3.1.3.5
UDP
-
Homo sapiens
3.1.3.5
UTP
-
Homo sapiens
3.1.3.5
XTP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.002
-
5'-CMP
-
Homo sapiens
3.1.3.5
0.014
-
5'-UMP
-
Homo sapiens
3.1.3.5
0.017
-
5'-dUMP
-
Homo sapiens
3.1.3.5
0.017
-
5'-GMP
-
Homo sapiens
3.1.3.5
0.018
-
5'-AMP
-
Homo sapiens
3.1.3.5
0.023
-
5'-dCMP
-
Homo sapiens
3.1.3.5
0.03
-
5'-IMP
-
Homo sapiens
3.1.3.5
0.035
-
5'-dAMP
-
Homo sapiens
3.1.3.5
0.044
-
5'-dGMP
-
Homo sapiens
3.1.3.5
0.057
-
5'-dIMP
-
Homo sapiens
3.1.3.5
0.065
-
5'-XMP
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytoplasm
two types of cytoplasmic enzymes: one type characterized by millimolar Km values, activation by ATP and ADP, regulation by phosphate. A second type characterized by micromolar Km values, inhibition by ATP, ADP and phosphate, the latter at high concentration
Homo sapiens
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95035
Homo sapiens
adenosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
-
95035
Homo sapiens
cytidine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95035
Homo sapiens
deoxyadenosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95035
Homo sapiens
deoxycytidine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95035
Homo sapiens
deoxyguanosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
95035
Homo sapiens
deoxyinosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
95035
Homo sapiens
deoxyuridne + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
-
95035
Homo sapiens
guanosine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-IMP + H2O
-
95035
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
-
95035
Homo sapiens
uridine + phosphate
-
95035
Homo sapiens
?
3.1.3.5
5'-XMP + H2O
-
95035
Homo sapiens
xanthosine + phosphate
-
95035
Homo sapiens
?