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Literature summary extracted from

  • Gil G.; Sitges M.; Hegardt F.G.
    Purification and properties of rat liver hydroxymethylglutaryl CoA reductase phosphatases (1981), Biochim. Biophys. Acta, 663, 211-221.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.47 fluoride
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.47 0.0000065
-
 [hydroxymethylglutaryl-CoA reductase]-phosphate for the cytosolic isoenzyme IIa Rattus norvegicus
3.1.3.47 0.000013
-
 [hydroxymethylglutaryl-CoA reductase]-phosphate for the cytosolic isoenzyme Ia Rattus norvegicus
3.1.3.47 0.0000195
-
 [hydroxymethylglutaryl-CoA reductase]-phosphate for the catalytic subunit of ca. 35000 Da Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.47 310000
-
 cytosolic isoenzyme IIa, gel chromatography Rattus norvegicus
3.1.3.47 480000
-
 cytosolic isoenzyme Ia, gel chromatography Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O Rattus norvegicus
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.3.47 Ethanol after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.47 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.47 liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.47 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.47 310 315 no comments on grade of purification, native cytosolic isoenzyme Ia Rattus norvegicus
3.1.3.47 670 710 no comments on grade of purification, native cytosolic isoenzyme IIa Rattus norvegicus
3.1.3.47 21480
-
 purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.3.47 -40°C, several months, no measurable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
 Rattus norvegicus [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
 ?
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.47 6 6.5 for cytosolic isoenzymes Ia and IIa Rattus norvegicus
3.1.3.47 6 6.5 for the catalytic subunit of ~35000 Da retrieved after ethanol treatment Rattus norvegicus