BRENDA - Enzyme Database show

Partial purification from rat liver microsomes of three native protein phosphatases with activity towards HMG-CoA reductase

Sitges M.; Gil G.; Hegardt G.; J. Lipid Res. 25, 497-506 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.47
diphosphate
-
Rattus norvegicus
3.1.3.47
ethanol
-
Rattus norvegicus
3.1.3.47
fluoride
-
Rattus norvegicus
3.1.3.47
phosphate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.47
cytosol
-
Rattus norvegicus
5829
-
3.1.3.47
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.47
75000
-
microsomal isoenzyme IM, gel chromatography
Rattus norvegicus
3.1.3.47
90000
-
microsomal isoenzyme Ex, gel chromatography
Rattus norvegicus
3.1.3.47
180000
-
microsomal isoenzyme IIM, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.47
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.47
liver
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.47
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.47
575
-
purification 55fold, for microsomal isoenzyme IM
Rattus norvegicus
3.1.3.47
1030
-
purification 98fold, for microsomal isoenzyme IIM
Rattus norvegicus
3.1.3.47
1375
-
purification 130fold, for microsomal isoenzyme Ex
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.47
-40░C, several months, no measurable loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94956
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94956
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94956
Rattus norvegicus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.47
6
-
for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
Rattus norvegicus
3.1.3.47
6.5
-
for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.47
diphosphate
-
Rattus norvegicus
3.1.3.47
ethanol
-
Rattus norvegicus
3.1.3.47
fluoride
-
Rattus norvegicus
3.1.3.47
phosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.47
cytosol
-
Rattus norvegicus
5829
-
3.1.3.47
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.47
75000
-
microsomal isoenzyme IM, gel chromatography
Rattus norvegicus
3.1.3.47
90000
-
microsomal isoenzyme Ex, gel chromatography
Rattus norvegicus
3.1.3.47
180000
-
microsomal isoenzyme IIM, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.47
liver
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.47
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.47
575
-
purification 55fold, for microsomal isoenzyme IM
Rattus norvegicus
3.1.3.47
1030
-
purification 98fold, for microsomal isoenzyme IIM
Rattus norvegicus
3.1.3.47
1375
-
purification 130fold, for microsomal isoenzyme Ex
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.47
-40░C, several months, no measurable loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94956
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94956
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94956
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.47
6
-
for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
Rattus norvegicus
3.1.3.47
6.5
-
for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
Rattus norvegicus