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Literature summary extracted from

  • Sitges M.; Gil G.; Hegardt G.
    Partial purification from rat liver microsomes of three native protein phosphatases with activity towards HMG-CoA reductase (1984), J. Lipid Res., 25, 497-506.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.47 diphosphate
-
 Rattus norvegicus
3.1.3.47 ethanol
-
 Rattus norvegicus
3.1.3.47 fluoride
-
 Rattus norvegicus
3.1.3.47 phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.47 cytosol
-
 Rattus norvegicus 5829
-
3.1.3.47 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.47 75000
-
 microsomal isoenzyme IM, gel chromatography Rattus norvegicus
3.1.3.47 90000
-
 microsomal isoenzyme Ex, gel chromatography Rattus norvegicus
3.1.3.47 180000
-
 microsomal isoenzyme IIM, gel chromatography Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.47 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.47 liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.47 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.47 575
-
 purification 55fold, for microsomal isoenzyme IM Rattus norvegicus
3.1.3.47 1030
-
 purification 98fold, for microsomal isoenzyme IIM Rattus norvegicus
3.1.3.47 1375
-
 purification 130fold, for microsomal isoenzyme Ex Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.3.47 -40°C, several months, no measurable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
 Rattus norvegicus [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
 ?
3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.47 6
-
 for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate Rattus norvegicus
3.1.3.47 6.5
-
 for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate Rattus norvegicus