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Literature summary extracted from

  • Pyko, M.; Rider, M.H.; Hue, L.; Wegener, G.
    6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase from frog skeletal muscle: purification, kinetics and immunological properties (1993), J. Comp. Physiol. B, 163, 89-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 Cyclic AMP dependent protein kinase
-
 Rattus norvegicus
3.1.3.46 Cyclic AMP dependent protein kinase
-
 Pelophylax lessonae
3.1.3.46 glycerol 1-phosphate
-
 Rattus norvegicus
3.1.3.46 glycerol 1-phosphate counteracts fructose 6-phosphate inhibition Pelophylax lessonae
3.1.3.46 MgGTP2-
-
 Pelophylax lessonae
3.1.3.46 phosphate
-
 Rattus norvegicus
3.1.3.46 phosphate counteracts inhibition by fructose-6-phosphate Pelophylax lessonae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.46 expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate strong Pelophylax lessonae
2.7.1.105 additional information no inhibition by phosphorylation with Ca2+/calmodulin dependent protein kinase; no inhibition by protein kinase C Pelophylax lessonae
2.7.1.105 phosphoenolpyruvate strong Pelophylax lessonae
2.7.1.105 sn-glycerol 3-phosphate not Pelophylax lessonae
3.1.3.46 fructose 6-phosphate
-
 Pelophylax lessonae
3.1.3.46 fructose 6-phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.46 0.0004
-
 fructose 2,6-bisphosphate
-
 Pelophylax lessonae
3.1.3.46 0.0006
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 53900
-
 2 * 53900, muscle-type isozyme, SDS-PAGE Pelophylax lessonae
2.7.1.105 55800
-
 2 * 55800, liver isoenzyme, SDS-PAGE Pelophylax lessonae
3.1.3.46 53300
-
 53300, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Pelophylax lessonae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Pelophylax lessonae
-
 frog
-
3.1.3.46 Pelophylax lessonae
-
-
-
3.1.3.46 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein
-
 Pelophylax lessonae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 2 isozymes, liver (L) and muscle (M) type Pelophylax lessonae
3.1.3.46 5600fold Pelophylax lessonae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Rattus norvegicus
a
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Pelophylax lessonae
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.46 muscle skeletal Rattus norvegicus
-
3.1.3.46 muscle skeletal Pelophylax lessonae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Pelophylax lessonae ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Pelophylax lessonae ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Pelophylax lessonae fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Pelophylax lessonae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 55800, liver isoenzyme, SDS-PAGE Pelophylax lessonae
2.7.1.105 dimer 2 * 53900, muscle-type isozyme, SDS-PAGE Pelophylax lessonae
3.1.3.46 dimer 53300, SDS-PAGE Rattus norvegicus
3.1.3.46 dimer 53900-55800, SDS-PAGE Pelophylax lessonae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 9.3
-
 muscle-type isozyme Pelophylax lessonae
3.1.3.46 5.5
-
-
 Rattus norvegicus
3.1.3.46 5.5
-
-
 Pelophylax lessonae