Literature summary extracted from

Kurland, I.J.; Pilkis, S.J.
Covalent control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: Insights into autoregulation of a bifunctional enzyme (1995), Protein Sci., 4, 1023-1037.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.3.46	Cyclic AMP dependent protein kinase	catalyzes phosphorylation at a single NH2-terminal residue, Ser-32	Rattus norvegicus
3.1.3.46	glycerol 1-phosphate	activates at saturating substrate concentrations	Rattus norvegicus
3.1.3.46	phosphate	activates at saturating substrate concentrations	Rattus norvegicus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.46	-	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.3.46	glycerol 1-phosphate	inhibits at subsaturating substrate concentrations	Rattus norvegicus
3.1.3.46	phosphate	inhibits at subsaturating substrate concentrations	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.1.3.46	fructose 2,6-bisphosphate + H2O	Rattus norvegicus	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.46	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.46	copurification with phosphofructokinase 2	Rattus norvegicus

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
3.1.3.46	beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate	bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase	Rattus norvegicus	a

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.3.46	liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.46	fructose 2,6-bisphosphate + H2O	-	Rattus norvegicus	fructose 6-phosphate + phosphate	-	?
3.1.3.46	fructose 2,6-bisphosphate + H2O	-	Rattus norvegicus	?	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1.3.46	0.1	-	fructose 2,6-bisphosphate	-	Rattus norvegicus

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Release 2023.1 (January 2023)