EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.46 | ATP | - |
Gallus gallus | |
3.1.3.46 | Cyclic AMP dependent protein kinase | activation | Gallus gallus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.105 | citrate | - |
Columba sp. | |
2.7.1.105 | citrate | - |
Gallus gallus | |
2.7.1.105 | additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Columba sp. | |
2.7.1.105 | additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Gallus gallus | |
3.1.3.46 | fructose 6-phosphate | - |
Gallus gallus | |
3.1.3.46 | fructose 6-phosphate | - |
Pigeon | |
3.1.3.46 | phosphate | - |
Gallus gallus | |
3.1.3.46 | phosphate | - |
Pigeon |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.105 | 0.0072 | - |
MgATP2- | pH 7.1, 30°C, phosphorylated enzyme | Columba sp. | |
2.7.1.105 | 0.0095 | 0.012 | MgATP2- | pH 7.1, 30°C, native pigeon enzyme | Columba sp. | |
2.7.1.105 | 0.05 | - |
beta-D-fructose 6-phosphate | pH 7.1, 30°C, MgATP2-, phosphorylated chicken enzyme | Gallus gallus | |
2.7.1.105 | 0.29 | - |
MgATP2- | pH 7.1, 30°C, native chicken enzyme | Gallus gallus | |
3.1.3.46 | 0.0001 | - |
fructose 2,6-bisphosphate | - |
Gallus gallus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.105 | phosphate | - |
Gallus gallus | |
2.7.1.105 | phosphate | - |
Columba sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.105 | 53000 | - |
2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 | Columba sp. |
2.7.1.105 | 54000 | - |
2 * 54000, SDS-PAGE | Gallus gallus |
2.7.1.105 | 110000 | - |
gel filtration | Gallus gallus |
2.7.1.105 | 110000 | - |
gel filtration | Columba sp. |
3.1.3.46 | 53000 | - |
2 * 53000, SDS-PAGE | Pigeon |
3.1.3.46 | 54000 | - |
2 * 54000, SDS-PAGE | Gallus gallus |
3.1.3.46 | 100000 | - |
gel filtration | Gallus gallus |
3.1.3.46 | 100000 | - |
gel filtration | Pigeon |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.46 | fructose 2,6-bisphosphate + H2O | Gallus gallus | - |
? | - |
? | |
3.1.3.46 | fructose 2,6-bisphosphate + H2O | Pigeon | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.105 | Columba sp. | - |
- |
- |
2.7.1.105 | Gallus gallus | - |
- |
- |
3.1.3.46 | Gallus gallus | - |
- |
- |
3.1.3.46 | Pigeon | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.1.105 | phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Gallus gallus |
2.7.1.105 | phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Columba sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.105 | - |
Gallus gallus |
2.7.1.105 | - |
Columba sp. |
3.1.3.46 | bifunctional enzyme, copurification with phosphofructokinase 2 | Gallus gallus |
3.1.3.46 | bifunctional enzyme, copurification with phosphofructokinase 2 | Pigeon |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.3.46 | beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Gallus gallus | |
3.1.3.46 | beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Pigeon |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.105 | liver | - |
Gallus gallus | - |
2.7.1.105 | muscle | - |
Columba sp. | - |
3.1.3.46 | liver | - |
Gallus gallus | - |
3.1.3.46 | muscle | breast | Gallus gallus | - |
3.1.3.46 | muscle | breast | Pigeon | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.105 | 0.11 | - |
- |
Columba sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.3.46 | -80°C, 20% glycerol, 200 mM KCl, 1 mM dithiothreitol, 20 mM Hepes, pH 7.1 | Gallus gallus |
3.1.3.46 | -80°C, 20% glycerol, 200 mM KCl, 1 mM dithiothreitol, 20 mM Hepes, pH 7.1 | Pigeon |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.105 | ATP + beta-D-fructose 6-phosphate | - |
Gallus gallus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
2.7.1.105 | ATP + beta-D-fructose 6-phosphate | - |
Columba sp. | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
2.7.1.105 | MgATP2- + beta-D-fructose 6-phosphate | - |
Gallus gallus | ? | - |
? | |
2.7.1.105 | MgATP2- + beta-D-fructose 6-phosphate | - |
Columba sp. | ? | - |
? | |
2.7.1.105 | additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Gallus gallus | ? | - |
? | |
2.7.1.105 | additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Columba sp. | ? | - |
? | |
3.1.3.46 | fructose 2,6-bisphosphate + H2O | - |
Gallus gallus | fructose 6-phosphate + phosphate | - |
? | |
3.1.3.46 | fructose 2,6-bisphosphate + H2O | - |
Pigeon | fructose 6-phosphate + phosphate | - |
? | |
3.1.3.46 | fructose 2,6-bisphosphate + H2O | - |
Gallus gallus | ? | - |
? | |
3.1.3.46 | fructose 2,6-bisphosphate + H2O | - |
Pigeon | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.105 | dimer | 2 * 54000, SDS-PAGE | Gallus gallus |
2.7.1.105 | dimer | 2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 | Columba sp. |
3.1.3.46 | dimer | 2 * 53000, SDS-PAGE | Pigeon |
3.1.3.46 | dimer | 2 * 54000, SDS-PAGE | Gallus gallus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.105 | 30 | - |
assay at | Gallus gallus |
2.7.1.105 | 30 | - |
assay at | Columba sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.105 | 10 | - |
- |
Gallus gallus |
2.7.1.105 | 10 | - |
- |
Columba sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.105 | 6 | 10 | about 80% of maximal activity at pH 6.0 and 9.5 | Gallus gallus |
2.7.1.105 | 7.5 | 10 | linear increase up to pH 10 from 10% of maximal activity at pH 7.5 | Columba sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.105 | 0.083 | - |
citrate | pH 7.1, 30°C, native enzyme | Columba sp. | |
2.7.1.105 | 0.084 | - |
citrate | pH 7.1, 30°C, phosphorylated enzyme | Columba sp. | |
2.7.1.105 | 0.13 | - |
citrate | pH 7.1, 30°C, phosphorylated enzyme | Gallus gallus | |
2.7.1.105 | 0.49 | - |
citrate | pH 7.1, 30°C, native enzyme | Gallus gallus |