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Literature summary extracted from

  • Van Schaftingen, E.; Hers, H.G.
    Purification and properties of phosphofructokinase 2/fructose 2,6-bisphosphatase from chicken liver and from pigeon muscle (1986), Eur. J. Biochem., 159, 359-365.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 ATP
-
Gallus gallus
3.1.3.46 Cyclic AMP dependent protein kinase activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate
-
Columba sp.
2.7.1.105 citrate
-
Gallus gallus
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Columba sp.
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Gallus gallus
3.1.3.46 fructose 6-phosphate
-
Gallus gallus
3.1.3.46 fructose 6-phosphate
-
Pigeon
3.1.3.46 phosphate
-
Gallus gallus
3.1.3.46 phosphate
-
Pigeon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.0072
-
MgATP2- pH 7.1, 30°C, phosphorylated enzyme Columba sp.
2.7.1.105 0.0095 0.012 MgATP2- pH 7.1, 30°C, native pigeon enzyme Columba sp.
2.7.1.105 0.05
-
beta-D-fructose 6-phosphate pH 7.1, 30°C, MgATP2-, phosphorylated chicken enzyme Gallus gallus
2.7.1.105 0.29
-
MgATP2- pH 7.1, 30°C, native chicken enzyme Gallus gallus
3.1.3.46 0.0001
-
fructose 2,6-bisphosphate
-
Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 phosphate
-
Gallus gallus
2.7.1.105 phosphate
-
Columba sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 53000
-
2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 Columba sp.
2.7.1.105 54000
-
2 * 54000, SDS-PAGE Gallus gallus
2.7.1.105 110000
-
gel filtration Gallus gallus
2.7.1.105 110000
-
gel filtration Columba sp.
3.1.3.46 53000
-
2 * 53000, SDS-PAGE Pigeon
3.1.3.46 54000
-
2 * 54000, SDS-PAGE Gallus gallus
3.1.3.46 100000
-
gel filtration Gallus gallus
3.1.3.46 100000
-
gel filtration Pigeon

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O Gallus gallus
-
?
-
?
3.1.3.46 fructose 2,6-bisphosphate + H2O Pigeon
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Columba sp.
-
-
-
2.7.1.105 Gallus gallus
-
-
-
3.1.3.46 Gallus gallus
-
-
-
3.1.3.46 Pigeon
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Gallus gallus
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Columba sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
Gallus gallus
2.7.1.105
-
Columba sp.
3.1.3.46 bifunctional enzyme, copurification with phosphofructokinase 2 Gallus gallus
3.1.3.46 bifunctional enzyme, copurification with phosphofructokinase 2 Pigeon

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Gallus gallus
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Pigeon

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
Gallus gallus
-
2.7.1.105 muscle
-
Columba sp.
-
3.1.3.46 liver
-
Gallus gallus
-
3.1.3.46 muscle breast Gallus gallus
-
3.1.3.46 muscle breast Pigeon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.11
-
-
Columba sp.

Storage Stability

EC Number Storage Stability Organism
3.1.3.46 -80°C, 20% glycerol, 200 mM KCl, 1 mM dithiothreitol, 20 mM Hepes, pH 7.1 Gallus gallus
3.1.3.46 -80°C, 20% glycerol, 200 mM KCl, 1 mM dithiothreitol, 20 mM Hepes, pH 7.1 Pigeon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
Gallus gallus ADP + beta-D-fructose 2,6-bisphosphate
-
r
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
Columba sp. ADP + beta-D-fructose 2,6-bisphosphate
-
r
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
Gallus gallus ?
-
?
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
Columba sp. ?
-
?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Gallus gallus ?
-
?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Columba sp. ?
-
?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
Gallus gallus fructose 6-phosphate + phosphate
-
?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
Pigeon fructose 6-phosphate + phosphate
-
?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
Gallus gallus ?
-
?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
Pigeon ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 54000, SDS-PAGE Gallus gallus
2.7.1.105 dimer 2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 Columba sp.
3.1.3.46 dimer 2 * 53000, SDS-PAGE Pigeon
3.1.3.46 dimer 2 * 54000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
assay at Gallus gallus
2.7.1.105 30
-
assay at Columba sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 10
-
-
Gallus gallus
2.7.1.105 10
-
-
Columba sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.105 6 10 about 80% of maximal activity at pH 6.0 and 9.5 Gallus gallus
2.7.1.105 7.5 10 linear increase up to pH 10 from 10% of maximal activity at pH 7.5 Columba sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 0.083
-
citrate pH 7.1, 30°C, native enzyme Columba sp.
2.7.1.105 0.084
-
citrate pH 7.1, 30°C, phosphorylated enzyme Columba sp.
2.7.1.105 0.13
-
citrate pH 7.1, 30°C, phosphorylated enzyme Gallus gallus
2.7.1.105 0.49
-
citrate pH 7.1, 30°C, native enzyme Gallus gallus