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Literature summary extracted from

  • Loiseau, A.M.; Rider, M.H.; Hue, L.
    Purification and properties of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase from rat hepatoma cells (1987), Biochem. Soc. Trans., 15, 384-385.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 Cyclic AMP dependent protein kinase activation Rattus norvegicus
3.1.3.46 glycerol 1-phosphate stimulates Rattus norvegicus
3.1.3.46 phosphate
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate
-
 Rattus norvegicus
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes 80% decrease in activity of the liver cells but not of hepatoma cells; phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Rattus norvegicus
2.7.1.105 phosphoenolpyruvate
-
 Rattus norvegicus
2.7.1.105 sn-glycerol 3-phosphate 75% decrease in activity of liver enzyme but not hepatoma cells Rattus norvegicus
3.1.3.46 fructose 6-phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information comparison of Km of hepatoma cells and liver enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ MgATP2 Rattus norvegicus
2.7.1.105 phosphate activation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Rattus norvegicus responsible for regulation of fructose 2,6-bisphosphate-concentration ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Rattus norvegicus
-
-
-
3.1.3.46 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes 80% decrease in activity of the liver cells but not of hepatoma cells Rattus norvegicus
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 hepatoma cell resembles muscle enzyme Rattus norvegicus
-
2.7.1.105 liver
-
 Rattus norvegicus
-
3.1.3.46 hepatoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate responsible for regulation of fructose 2,6-bisphosphate-concentration Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 0.025
-
 citrate hepatoma cells Rattus norvegicus
2.7.1.105 0.035
-
 citrate
-
 Rattus norvegicus
2.7.1.105 0.12
-
 phosphoenolpyruvate hepatoma cells Rattus norvegicus
2.7.1.105 0.2
-
 phosphoenolpyruvate liver Rattus norvegicus
2.7.1.105 0.2
-
 citrate liver Rattus norvegicus