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Literature summary extracted from

  • Walker, G.H.; Huber, S.C.
    ATP-dependent activation of a new form of spinach leaf 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (1987), Arch. Biochem. Biophys., 258, 58-64.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 additional information one enzyme form is activated dramatically as result of modification by MgATP during preincubation Spinacia oleracea
3.1.3.46 MgATP2-
-
 Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.46 fructose 6-phosphate
-
 Spinacia oleracea
3.1.3.46 phosphate
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.46 0.0001
-
 fructose 2,6-bisphosphate
-
 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+
-
 Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Spinacia oleracea enzyme plays an important role in the regulation of hepatic carbohydrate metabolism ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Spinacia oleracea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Spinacia oleracea
-
-
-
3.1.3.46 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 2 distinct molecular forms Spinacia oleracea
2.7.1.105 partial Spinacia oleracea
3.1.3.46 two isoenzymes, partially purified Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Spinacia oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 leaf
-
 Spinacia oleracea
-
3.1.3.46 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.105 -80°C, partially purified, less than 10% loss of activity within 1 month Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Spinacia oleracea ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate enzyme plays an important role in the regulation of hepatic carbohydrate metabolism Spinacia oleracea ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Spinacia oleracea fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Spinacia oleracea ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 25
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 7.4 7.9
-
 Spinacia oleracea
2.7.1.105 7.6
-
-
 Spinacia oleracea
3.1.3.46 7
-
-
 Spinacia oleracea
3.1.3.46 7.6
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.105 7 8.2 about half-maximal activity at pH 7.0 and 8.2, peak II enzyme Spinacia oleracea
2.7.1.105 7.1 8.3 about half-maximal activity at pH 7.1 and 8.3, peak 1 enzyme Spinacia oleracea