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Literature summary extracted from

  • Macdonald, F.D.; Chou, Q.; Buchanan, B.B.; Stitt, M.
    Purification and characterization of fructose-2,6-bisphosphatase, a substrate-specific cytosolic enzyme from leaves (1989), J. Biol. Chem., 264, 5540-5544.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 EDTA
-
 Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.46 AMP
-
 Spinacia oleracea
3.1.3.46 fructose 6-phosphate
-
 Spinacia oleracea
3.1.3.46 MgCl2
-
 Spinacia oleracea
3.1.3.46 phosphate
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.46 0.032
-
 fructose 2,6-bisphosphate
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.46 cytosol
-
 Spinacia oleracea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.46 33000
-
 2 * 33000, SDS-PAGE Spinacia oleracea
3.1.3.46 76200
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O Spinacia oleracea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.46 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.46 10000fold Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Spinacia oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.46 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Spinacia oleracea fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.46 dimer 2 * 33000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.46 7
-
 pretreatment with MgATP2-, form 1 Spinacia oleracea
3.1.3.46 7.3
-
-
 Spinacia oleracea
3.1.3.46 7.6
-
 pretreatment with MgATP2-, form 2 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.46 6.5 8
-
 Spinacia oleracea