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Literature summary extracted from

  • Roche, T.E.; Cate, R.L.
    Purification of porcine liver pyruvate dehydrogenase complex and characterization of its catalytic and regulatory properties (1977), Arch. Biochem. Biophys., 183, 664-677.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.43 rabbit serum stabilizes Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.43 EGTA with Mg2+ as cofactor Sus scrofa
3.1.3.43 NADH 0.5 mM with 10 mM Mg2+ and 0.1 mM Ca2+ Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 0.25
-
 pyruvate
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 mitochondrion
-
 Sus scrofa 5739
-
3.1.3.43 mitochondrion
-
 Sus scrofa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.43 Ca2+ Km: 0.001-0.003 mM Sus scrofa
3.1.3.43 Ca2+ overcomes EGTA inhibition Sus scrofa
3.1.3.43 KCl
-
 Sus scrofa
3.1.3.43 Mg2+ Km: 4.2 mM Sus scrofa
3.1.3.43 Mn2+ Km: 1 mM Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Sus scrofa
-
-
-
3.1.3.43 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Sus scrofa
3.1.3.43 partially Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.104 liver
-
 Sus scrofa
-
3.1.3.43 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Sus scrofa [pyruvate dehydrogenase (lipoamide)] + phosphate
-
 ir
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Sus scrofa ?
-
 ?