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Literature summary extracted from
- Purification of bovine kidney and heart pyruvate dehydrogenase phosphatase on Sepharose derivatized with the pyruvate dehydrogenase complex (1982), Eur. J. Biochem., 125, 349-355.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.43 | mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.43 | Ca2+ | - |
Bos taurus |  |
| 3.1.3.43 | Mg2+ | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.43 | 49000 | - |
1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer | Bos taurus |
| 3.1.3.43 | 89000 | - |
1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer | Bos taurus |
| 3.1.3.43 | 140000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.43 | [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | Bos taurus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.43 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.43 | assay method | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.43 | heart | - |
Bos taurus | - |
| 3.1.3.43 | kidney | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.43 | -70°C, 400 mM KCl for 2 months no loss of activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.43 | [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Bos taurus | [pyruvate dehydrogenase (lipoamide)] + phosphate | - |
ir | |
| 3.1.3.43 | [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.43 | dimer | 1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer | Bos taurus |
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Release 2023.1 (January 2023)
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