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Literature summary extracted from

  • Reed, L.J.; Yeaman, S.J.
    Pyruvate dehydrogenase (1987), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 18, 77-95.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.43 NAD+ reverses NADH inhibition Bos taurus
3.1.3.43 putrescine
-
 Bos taurus
3.1.3.43 spermidine
-
 Bos taurus
3.1.3.43 spermine in presence of Mg2+ and Ca2+ Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 phosphorylation
-
 Bos taurus
3.1.3.43 KCl
-
 Bos taurus
3.1.3.43 NADH
-
 Bos taurus
3.1.3.43 p-nitrophenylphosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.43 0.0029
-
 [Pyruvate dehydrogenase (lipoamide)]-phosphate with Ca2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 mitochondrion located within the inner membrane matrix compartment Bos taurus 5739
-
3.1.3.43 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.104 Ca2+ activates mammalian pyruvate dehydrogenase phosphatase Bos taurus
1.2.1.104 Mg2+ activates mammalian pyruvate dehydrogenase phosphatase Bos taurus
3.1.3.43 Ca2+
-
 Bos taurus
3.1.3.43 Mg2+ Km: 2 mM Bos taurus
3.1.3.43 Mn2+
-
 Bos taurus
3.1.3.43 Mn2+ Km: 0.5 mM Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.104 36000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 41000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 50000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 52000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 55000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 154000
-
-
 Bos taurus
2.3.1.12 52000
-
 60 * 52000 Mammalia
2.3.1.12 3100000
-
-
 Mammalia
3.1.3.43 50000
-
 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus
3.1.3.43 97000
-
 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus
3.1.3.43 150000
-
 sedimentation equilibrium and gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Mammalia
-
 S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Geobacillus stearothermophilus
-
 S-acetyldihydrolipoamide + CoA
-
 ?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Bos taurus
-
 ?
-
 ?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Neurospora crassa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Bos taurus
-
-
-
2.3.1.12 Geobacillus stearothermophilus
-
-
-
2.3.1.12 Mammalia
-
-
-
3.1.3.43 Bos taurus
-
 overview
-
3.1.3.43 Neurospora crassa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.104 side-chain modification reversible posphorylation of mammalian alpha subunit by pyruvate dehydrogenase kinase at three serine residues inactivates enzyme, dephosphorylation by phosphatase activates Bos taurus
2.3.1.12 lipoprotein 1 lipoyl domain Mammalia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.104 heart
-
 Bos taurus
-
1.2.1.104 kidney
-
 Bos taurus
-
3.1.3.43 heart
-
 Bos taurus
-
3.1.3.43 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Mammalia S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Geobacillus stearothermophilus S-acetyldihydrolipoamide + CoA
-
 ?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Bos taurus [pyruvate dehydrogenase (lipoamide)] + phosphate
-
 ir
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Neurospora crassa [pyruvate dehydrogenase (lipoamide)] + phosphate
-
 ir
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Bos taurus ?
-
 ?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.104 oligomer 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 tetramer
-
 Bos taurus
2.3.1.12 polymer 60 * 52000 Mammalia
2.3.1.12 polymer stoichiometry of pyruvate dehydrogenase complexes Mammalia
2.3.1.12 polymer stoichiometry of pyruvate dehydrogenase complexes Geobacillus stearothermophilus
2.3.1.12 polymer 60 subunits Geobacillus stearothermophilus
3.1.3.43 dimer 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus