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Literature summary extracted from

  • Larson, T.J.; Hirabayashi, T.; Dowhan, W.
    Phosphatidylglycerol biosynthesis in Bacillus licheniformis. Resolution of membrane-bound enzymes by affinity chromatography on cytidinediphospho-sn-1,2-diacylglycerol sepharose (1976), Biochemistry, 15, 974-979.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.5 Triton X-100 maximal activity is dependent on Triton X-100 Bacillus licheniformis
3.1.3.27 Triton X-100
-
 Bacillus licheniformis

General Stability

EC Number General Stability Organism
3.1.3.27 50% loss of activity after solubilization with Triton X-100 in 12 h Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.27 Triton X-100 high concentration Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.5 additional information
-
 additional information anomalous kinetics for CDP-diacylglycerol Bacillus licheniformis
2.7.8.5 0.15
-
 sn-glycerol 3-phosphate 30°C, pH 7.0 Bacillus licheniformis
3.1.3.27 0.18
-
 phosphatidylglycerophosphate
-
 Bacillus licheniformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.5 membrane bound Bacillus licheniformis 16020
-
3.1.3.27 cytoplasmic membrane
-
 Bacillus licheniformis
-
-
3.1.3.27 membrane
-
 Bacillus licheniformis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.5 Ca2+ cannot substitute for Mg2+ in activation Bacillus licheniformis
2.7.8.5 Mg2+ 80 mM required for maximal activity Bacillus licheniformis
2.7.8.5 Mn2+ cannot substitute for Mg2+ Bacillus licheniformis
3.1.3.27 Mg2+ addition of high concentrated divalent cations not required Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.27 phosphatidylglycerophosphate + H2O Bacillus licheniformis biosynthesis of phospholipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.5 Bacillus licheniformis
-
-
-
3.1.3.27 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.5 partial Bacillus licheniformis
3.1.3.27 partial Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.5 0.36
-
 pH 7.0, 30°C Bacillus licheniformis
3.1.3.27 0.0023
-
 partially purified Bacillus licheniformis

Storage Stability

EC Number Storage Stability Organism
2.7.8.5 4°C, 50% loss of activity after 12 h, broken cell preparation Bacillus licheniformis
3.1.3.27 4°C, for weeks in 2% Triton X-100, 50 mM MgCl2, 20% glycerol, 0.5 M KCl Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.5 CDP-diacylglycerol + sn-glycero-3-phosphate
-
 Bacillus licheniformis CMP + phosphatidylglycerophosphate 1-(3-glycerophosphoryl)-glycerol 3-phosphate ?
3.1.3.27 phosphatidylglycerophosphate + H2O
-
 Bacillus licheniformis 3-phosphatidylglycerol + phosphate
-
 ?
3.1.3.27 phosphatidylglycerophosphate + H2O biosynthesis of phospholipids Bacillus licheniformis ?
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.8.5 30
-
 assay at Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.27 6 8.5
-
 Bacillus licheniformis