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Literature summary extracted from

  • Nair, P.M.
    Asparagine synthetase from gamma-irradiated potatoes (1969), Arch. Biochem. Biophys., 133, 208-215.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.4 Asn
-
 Solanum tuberosum
6.3.1.4 CuSO4
-
 Solanum tuberosum
6.3.1.4 EDTA
-
 Solanum tuberosum
6.3.1.4 hydroxylamine above 400 mM Solanum tuberosum
6.3.1.4 MoO42-
-
 Solanum tuberosum
6.3.1.4 Na2HAsO4
-
 Solanum tuberosum
6.3.1.4 NaF
-
 Solanum tuberosum
6.3.1.4 p-chloromercuribenzoate
-
 Solanum tuberosum
6.3.1.4 ZnSO4
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.4 10
-
 ATP
-
 Solanum tuberosum
6.3.1.4 26
-
 L-Asp
-
 Solanum tuberosum
6.3.1.4 40
-
 NH4+
-
 Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.4 Mg2+ required Solanum tuberosum
6.3.1.4 Mg2+ Km: 4 mM Solanum tuberosum
6.3.1.4 Mn2+ can replace Mg2+ Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.4 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.4
-
 Solanum tuberosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.4 0.0583
-
-
 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
6.3.1.4 -20°C, stable for 1 week Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.4 ATP + L-Asp + hydroxylamine maximal activity at 400 mM NH2OH Solanum tuberosum ADP + phosphate + beta-aspartylhydroxamate
-
 ?
6.3.1.4 ATP + L-Asp + NH4+
-
 Solanum tuberosum ADP + phosphate + L-Asn
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.4 6
-
 Mn2+-stimulated reaction Solanum tuberosum
6.3.1.4 7.2
-
 Mg2+-stimulated reaction Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.4 5.2 8 5.2: about 70% of maximal Mn2+-stimulated activity, 8.0: about 35% of maximal Mn2+-stimulated activity Solanum tuberosum
6.3.1.4 6 8.4 6.0: about 70% of maximal Mg2+-stimulated activity, 8.4: about 60% of maximal Mg2+-stimulated activity Solanum tuberosum