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Literature summary extracted from

  • Goel, M.; Sharma, C.B.
    Multiple forms of phytases in germinating cotyledons of Cucurbita maxima (1979), Phytochemistry, 18, 1939-1942.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 F-
-
 Cucurbita maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 1.5
-
 myo-inositol hexakisphosphate phytase II Cucurbita maxima
3.1.3.26 2.2
-
 myo-inositol hexakisphosphate phytase IC Cucurbita maxima
3.1.3.26 2.8
-
 myo-inositol hexakisphosphate phytase IB Cucurbita maxima
3.1.3.26 4.5
-
 myo-inositol hexakisphosphate phytase ID Cucurbita maxima
3.1.3.26 6.7
-
 myo-inositol hexakisphosphate phytase IA Cucurbita maxima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 32400
-
 phytase II, gel filtration Cucurbita maxima
3.1.3.26 66500
-
 phytase IA, IB, IC and ID, gel filtration Cucurbita maxima

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Cucurbita maxima
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Cucurbita maxima
-
 multiple forms: phytase IA, IB, IC, ID and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26
-
 Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 cotyledon germinating Cucurbita maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 additional information
-
-
 Cucurbita maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Cucurbita maxima myo-inositol pentakisphosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 60
-
 60 min: 40% loss of activity of phytase IA, 30% loss of activity of phytase IB, 25% loss of activity of phytase IC, 30% loss of activity of phytase ID, 10% loss of activity of phytase II Cucurbita maxima
3.1.3.26 70
-
 60 min: 65% loss of activity of phytase IA, 55% loss of activity of phytase IB, 60% loss of activity of phytase IC, 58% loss of activity of phytase ID, 40% loss of activity of phytase II Cucurbita maxima
3.1.3.26 80
-
 60 min: 90% loss of activity of phytase IA, 80% loss of activity of phytase IB, 92% loss of activity of phytase IC, 88% loss of activity of phytase ID, 80% loss of activity of phytase II Cucurbita maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.8
-
 phytase IA, and IB Cucurbita maxima
3.1.3.26 5.2
-
 phytase IC and ID Cucurbita maxima
3.1.3.26 5.5
-
 phytase II Cucurbita maxima