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Literature summary extracted from

  • Houde, R.L.; Alli, I.; Kermasha, S.
    Purification and characterization of Canola seed (Brassica sp.) phytase (1990), J. Food Biochem., 14, 331-351.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 Cu2+ strong Brassica sp.
3.1.3.26 Pb2+
-
 Brassica sp.
3.1.3.26 phosphate competitive Brassica sp.
3.1.3.26 Zn2+
-
 Brassica sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.36
-
 myo-inositol hexakisphosphate
-
 Brassica sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Co2+ 0.1 mM, activation Brassica sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Brassica sp.
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 partial Brassica sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 seed
-
 Brassica sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 ATP + H2O
-
 Brassica sp. ?
-
 ?
3.1.3.26 diphosphate + H2O
-
 Brassica sp. 2 phosphate
-
 ?
3.1.3.26 glycerol 2-phosphate + H2O
-
 Brassica sp. glycerol + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Brassica sp. myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
 Brassica sp. p-nitrophenol + phosphate
-
 ?
3.1.3.26 phenyl phosphate + H2O
-
 Brassica sp. phenol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 50
-
-
 Brassica sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 5.2
-
-
 Brassica sp.