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Literature summary extracted from

  • Greiner, R.; Alminger, M.L.
    Purification and characterization of a phytate-degrading enzyme from germinated oat (Avena sativa) (1999), J. Sci. Food Agric., 79, 1453-1460.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 Cu2+ 0.1-1 mM, strong Avena sativa
3.1.3.26 F-
-
 Avena sativa
3.1.3.26 Fe2+ 0.1-1 mM, strong Avena sativa
3.1.3.26 Fe3+ 0.1-1 mM, strong Avena sativa
3.1.3.26 Hg2+ 0.1-1 mM, strong Avena sativa
3.1.3.26 molybdate
-
 Avena sativa
3.1.3.26 phosphate
-
 Avena sativa
3.1.3.26 vanadate
-
 Avena sativa
3.1.3.26 wolframate
-
 Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.03
-
 myo-inositol hexakisphosphate
-
 Avena sativa
3.1.3.26 0.045
-
 fructose 6-phosphate
-
 Avena sativa
3.1.3.26 0.045
-
 pyridoxal phosphate
-
 Avena sativa
3.1.3.26 0.067
-
 glucose 6-phosphate
-
 Avena sativa
3.1.3.26 0.075
-
 Glycerol 2-phosphate
-
 Avena sativa
3.1.3.26 0.09
-
 fructose 1,6-diphosphate
-
 Avena sativa
3.1.3.26 0.093
-
 O-phospho-L-Ser
-
 Avena sativa
3.1.3.26 0.105
-
 diphosphate
-
 Avena sativa
3.1.3.26 0.115
-
 AMP
-
 Avena sativa
3.1.3.26 0.132
-
 p-nitrophenyl phosphate
-
 Avena sativa
3.1.3.26 0.142
-
 ADP
-
 Avena sativa
3.1.3.26 0.145
-
 ATP
-
 Avena sativa
3.1.3.26 0.15
-
 2-naphthyl phosphate
-
 Avena sativa
3.1.3.26 0.153
-
 GTP
-
 Avena sativa
3.1.3.26 0.157
-
 1-naphthyl phosphate
-
 Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 67000
-
 gel filtration Avena sativa
3.1.3.26 68000
-
 1 * 68000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.26 additional information Avena sativa highest activity in cells in the late stationary phase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Avena sativa
-
 cv Sang
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 enzyme from cells in the late stationary phase Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 seedling germinated Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 307
-
-
 Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 1-naphthyl phosphate + H2O
-
 Avena sativa 1-naphthol + phosphate
-
 ?
3.1.3.26 2-naphthyl phosphate + H2O
-
 Avena sativa 2-naphthol + phosphate
-
 ?
3.1.3.26 ADP + H2O
-
 Avena sativa ?
-
 ?
3.1.3.26 AMP + H2O
-
 Avena sativa adenosine + phosphate
-
 ?
3.1.3.26 ATP + H2O
-
 Avena sativa ?
-
 ?
3.1.3.26 D-fructose 6-phosphate + H2O
-
 Avena sativa D-fructose + phosphate
-
 ?
3.1.3.26 diphosphate + H2O
-
 Avena sativa 2 phosphate
-
 ?
3.1.3.26 fructose 1,6-diphosphate + H2O
-
 Avena sativa ?
-
 ?
3.1.3.26 Glucose 6-phosphate + H2O
-
 Avena sativa Glucose + phosphate
-
 ?
3.1.3.26 glycerol 2-phosphate + H2O
-
 Avena sativa glycerol + phosphate
-
 ?
3.1.3.26 GTP + H2O
-
 Avena sativa ?
-
 ?
3.1.3.26 additional information highest activity in cells in the late stationary phase Avena sativa ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Avena sativa myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 NADP+ + H2O
-
 Avena sativa NAD+ + phosphate
-
 ?
3.1.3.26 O-phospho-L-Ser + H2O
-
 Avena sativa Ser + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
 Avena sativa p-nitrophenol + phosphate
-
 ?
3.1.3.26 pyridoxal phosphate + H2O
-
 Avena sativa pyridoxal + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 monomer 1 * 68000, SDS-PAGE Avena sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 38
-
-
 Avena sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 35
-
 90 min, stable Avena sativa
3.1.3.26 40
-
 90 min, 15% loss of activity Avena sativa
3.1.3.26 50
-
 4°C, 90 min, 61% loss of activity Avena sativa
3.1.3.26 60
-
 4°C, 90 min, complete inactivation Avena sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.26 5
-
 O-phospho-L-Ser
-
 Avena sativa
3.1.3.26 16
-
 AMP
-
 Avena sativa
3.1.3.26 17
-
 pyridoxal phosphate
-
 Avena sativa
3.1.3.26 35
-
 fructose 6-phosphate
-
 Avena sativa
3.1.3.26 70
-
 glucose 6-phosphate
-
 Avena sativa
3.1.3.26 102
-
 Glycerol 2-phosphate
-
 Avena sativa
3.1.3.26 210
-
 fructose 1,6-diphosphate
-
 Avena sativa
3.1.3.26 305
-
 1-naphthyl phosphate
-
 Avena sativa
3.1.3.26 356
-
 myo-inositol hexakisphosphate
-
 Avena sativa
3.1.3.26 360
-
 2-naphthyl phosphate
-
 Avena sativa
3.1.3.26 375
-
 p-nitrophenyl phosphate
-
 Avena sativa
3.1.3.26 388
-
 GTP
-
 Avena sativa
3.1.3.26 450
-
 ADP
-
 Avena sativa
3.1.3.26 645
-
 ATP
-
 Avena sativa
3.1.3.26 943
-
 diphosphate
-
 Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 5
-
-
 Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 2.5 7.5 inactive below pH 2.5 and above pH 7.5 Avena sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.26 2.5
-
 4°C, 5 days, 78% loss of original activity Avena sativa
3.1.3.26 3.5 7.5 stable Avena sativa
3.1.3.26 8.5
-
 4°C, 5 days, 78% loss of original activity Avena sativa