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Literature summary extracted from

  • Ye, J.J.; Saier, M.H.
    Purification and characterization of a small membrane-associated sugar phosphate phosphatase that is allosterically activated by HPr(Ser(P)) of the phosphotransferase system in Lactococcus lactis (1995), J. Biol. Chem., 270, 16740-16744.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.23 S46D HPr i.e. mutant phosphocarrier protein, 10fold activation of sugar phosphate phosphatase I Lactococcus lactis
3.1.3.23 S46D HPr Km: 0.007 mM Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.23 50
-
 mannitol 1-phosphate
-
 Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.23 membrane associated Lactococcus lactis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.23 Co2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
3.1.3.23 Fe2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
3.1.3.23 Mg2+ the enzyme is dependent on divalent cations, Mg2+ is most effective, half-maximal stimulation at 1 mM Lactococcus lactis
3.1.3.23 Mn2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
3.1.3.23 Zn2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.23 Lactococcus lactis
-
 sugar phosphate phosphatase II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.23 2,3-diphosphoglycerate + H2O
-
 Lactococcus lactis ?
-
 ?
3.1.3.23 2-Deoxy-D-glucose 6-phosphate + H2O
-
 Lactococcus lactis 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.23 alpha-D-glucose 1-phosphate + H2O
-
 Lactococcus lactis alpha-D-glucose + phosphate
-
 ?
3.1.3.23 beta-D-methyl-thiogalactopyranoside 6-phosphate + H2O
-
 Lactococcus lactis beta-D-methyl-thiogalactopyranoside + phosphate
-
 ?
3.1.3.23 D-fructose 1-phosphate + H2O
-
 Lactococcus lactis D-fructose + phosphate
-
 ?
3.1.3.23 D-fructose 6-phosphate + H2O
-
 Lactococcus lactis D-fructose + phosphate
-
 ?
3.1.3.23 D-galactose 6-phosphate + H2O
-
 Lactococcus lactis D-galactose + phosphate
-
 ?
3.1.3.23 D-glucose 6-phosphate + H2O
-
 Lactococcus lactis D-glucose + phosphate
-
 ?
3.1.3.23 D-mannose 6-phosphate + H2O
-
 Lactococcus lactis D-mannose + phosphate
-
 ?
3.1.3.23 mannitol 1-phosphate + H2O
-
 Lactococcus lactis mannitol + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.23 100
-
 10 min, unaffected Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.23 7 8 hydrolysis of mannitol 1-phosphate, with or without the activator S46D HPr Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.23 5 9 about 60% of maximal activity at pH 5.0 and at pH 9.0, hydrolysis of mannitol 1-phosphate Lactococcus lactis