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Literature summary extracted from
- Molecular weight and some physicochemical and catalytic properties of sugar phosphate phosphohydrolase from Escherichia coli and Neisseria meningitidis (1975), J. Biol. Chem., 250, 3729-3737.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.3.23 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.23 | Dodecyl sulfate | - |
Escherichia coli |  |
| 3.1.3.23 | Dodecyl sulfate | - |
Neisseria meningitidis | |
| 3.1.3.23 | glucose 1-phosphate | complete inhibition of hydrolysis of glucose 6-phosphate as long as glucose 1-phosphate to glucose 6-phosphate ratios are greater than 0.12 | Escherichia coli | |
| 3.1.3.23 | glucose 1-phosphate | complete inhibition of hydrolysis of glucose 6-phosphate as long as glucose 1-phosphate to glucose 6-phosphate ratios are greater than 0.12 | Neisseria meningitidis | |
| 3.1.3.23 | guanidine | - |
Escherichia coli | |
| 3.1.3.23 | guanidine | - |
Neisseria meningitidis | |
| 3.1.3.23 | mannose 1-phosphate | strong competitive inhibitor of hydrolysis of glucose 6-phosphate | Escherichia coli | |
| 3.1.3.23 | p-mercuribenzoate | - |
Escherichia coli | |
| 3.1.3.23 | p-mercuribenzoate | - |
Neisseria meningitidis | |
| 3.1.3.23 | Urea | - |
Escherichia coli | |
| 3.1.3.23 | Urea | - |
Neisseria meningitidis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.23 | 1.2 | - |
D-glucuronate 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.1 | - |
alpha-D-xylose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.2 | - |
alpha-D-gentiobiose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.2 | - |
alpha-D-maltose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.3 | - |
alpha-D-lactose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.5 | - |
alpha-D-glucose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.5 | - |
alpha-D-galactose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.5 | - |
alpha-D-mannose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 2.8 | - |
D-Fructose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3 | - |
D-fructose 6-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3 | - |
D-glucosamine 6-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3 | - |
alpha-D-mannose 1-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3.1 | - |
D-glucose 6-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3.1 | - |
2-deoxy-D-glucose 6-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3.1 | - |
D-galactose 6-phosphate | - |
Escherichia coli | |
| 3.1.3.23 | 3.3 | - |
D-ribose 5-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 51000 | - |
2 * 51000, SDS-PAGE | Escherichia coli |
| 3.1.3.23 | 100000 | - |
gel filtration | Escherichia coli |
| 3.1.3.23 | 100000 | - |
gel filtration | Neisseria meningitidis |
| 3.1.3.23 | 100000 | - |
low speed sedimentation | Neisseria meningitidis |
| 3.1.3.23 | 101000 | - |
low speed sedimentation | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.23 | Escherichia coli | - |
- |
- |
| 3.1.3.23 | Neisseria meningitidis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.23 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 7.81 | - |
- |
Neisseria meningitidis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.23 | 1-3°C, pH 10, stable for 12 weeks | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.23 | 2-Deoxy-D-glucose 6-phosphate + H2O | - |
Escherichia coli | 2-Deoxy-D-glucose + phosphate | - |
? | |
| 3.1.3.23 | 2-Deoxy-D-glucose 6-phosphate + H2O | - |
Neisseria meningitidis | 2-Deoxy-D-glucose + phosphate | - |
? | |
| 3.1.3.23 | alpha-cellobiose 1-phosphate + H2O | - |
Escherichia coli | alpha-cellobiose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-galactose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-galactose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-galactose 1-phosphate + H2O | - |
Neisseria meningitidis | alpha-D-galactose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-gentiobiose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-gentiobiose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-glucosamine 1-phosphate + H2O | - |
Escherichia coli | alpha-D-glucosamine + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-glucose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-glucose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-glucose 1-phosphate + H2O | - |
Neisseria meningitidis | alpha-D-glucose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-glucuronic acid 1-phosphate + H2O | - |
Escherichia coli | alpha-D-glucuronic acid + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-glucuronic acid 1-phosphate + H2O | - |
Neisseria meningitidis | alpha-D-glucuronic acid + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-lactose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-lactose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-mannose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-mannose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-mannose 1-phosphate + H2O | - |
Neisseria meningitidis | alpha-D-mannose + phosphate | - |
? | |
| 3.1.3.23 | alpha-D-xylose 1-phosphate + H2O | - |
Escherichia coli | alpha-D-xylose + phosphate | - |
? | |
| 3.1.3.23 | alpha-gentiobiose 1-phosphate + H2O | - |
Escherichia coli | alpha-gentiobiose + phosphate | - |
? | |
| 3.1.3.23 | alpha-lactose 1-phosphate + H2O | - |
Escherichia coli | alpha-lactose + phosphate | - |
? | |
| 3.1.3.23 | alpha-lactose 1-phosphate + H2O | - |
Neisseria meningitidis | alpha-lactose + phosphate | - |
? | |
| 3.1.3.23 | alpha-maltose 1-phosphate + H2O | - |
Escherichia coli | alpha-maltose + phosphate | - |
? | |
| 3.1.3.23 | D-fructose 1,6-diphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.1.3.23 | D-fructose 1,6-diphosphate + H2O | - |
Neisseria meningitidis | ? | - |
? | |
| 3.1.3.23 | D-fructose 1-phosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| 3.1.3.23 | D-fructose 1-phosphate + H2O | - |
Neisseria meningitidis | D-fructose + phosphate | - |
? | |
| 3.1.3.23 | D-fructose 6-phosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| 3.1.3.23 | D-fructose 6-phosphate + H2O | - |
Neisseria meningitidis | D-fructose + phosphate | - |
? | |
| 3.1.3.23 | D-galactose 6-phosphate + H2O | - |
Escherichia coli | D-galactose + phosphate | - |
? | |
| 3.1.3.23 | D-galactose 6-phosphate + H2O | - |
Neisseria meningitidis | D-galactose + phosphate | - |
? | |
| 3.1.3.23 | D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-glucosamine + phosphate | - |
? | |
| 3.1.3.23 | D-glucose 6-phosphate + H2O | - |
Escherichia coli | D-glucose + phosphate | - |
? | |
| 3.1.3.23 | D-glucose 6-phosphate + H2O | - |
Neisseria meningitidis | D-glucose + phosphate | - |
? | |
| 3.1.3.23 | D-glucuronate 1-phosphate + H2O | - |
Escherichia coli | D-glucuronate + phosphate | - |
? | |
| 3.1.3.23 | D-mannose 6-phosphate + H2O | - |
Escherichia coli | D-mannose + phosphate | - |
? | |
| 3.1.3.23 | D-mannose 6-phosphate + H2O | - |
Neisseria meningitidis | D-mannose + phosphate | - |
? | |
| 3.1.3.23 | D-ribose 5-phosphate + H2O | - |
Escherichia coli | D-ribose + phosphate | - |
? | |
| 3.1.3.23 | D-ribose 5-phosphate + H2O | - |
Neisseria meningitidis | D-ribose + phosphate | - |
? | |
| 3.1.3.23 | D-xylose 1-phosphate + H2O | - |
Escherichia coli | D-xylose + phosphate | - |
? | |
| 3.1.3.23 | D-xylose 1-phosphate + H2O | - |
Neisseria meningitidis | D-xylose + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.23 | dimer | 2 * 51000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 30 | - |
pH 3.0-9.7, stable for at least 40 min, not stable above pH 10 or below pH 2.5 | Escherichia coli |
| 3.1.3.23 | 55 | - |
pH 6, 2 min, stable up to | Escherichia coli |
| 3.1.3.23 | 60 | - |
pH 4.0-9.0, 2 min, quite stable. At pH 6.0, 20% loss of activity after 2 min. Complete loss of activity at pH 1.0, 2.0, 3.0 or 10.0 after 2 min | Escherichia coli |
| 3.1.3.23 | 65 | - |
pH 6.0, 2 min, 80% loss of activity | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 5.9 | - |
hydrolysis of glucose 1-phosphate or glucose 6-phosphate | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 4 | 8 | pH 4.0: about 55% of maximal activity, pH 8.0: about 45% of maximal activity, hydrolysis of glucose 1 -phosphate | Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.23 | 3 | 9.7 | 30°C, stable for at least 40 min | Escherichia coli |
| 3.1.3.23 | 4 | 9 | 60°C, 2 min, quite stable | Escherichia coli |
| 3.1.3.23 | 6 | - |
stable up to 55°C. At 60°C, 2 min, about 20% loss of activity. At 65°C, 2 min, 80% loss of activity | Escherichia coli |
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