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Literature summary extracted from

  • Liberator, P.; Anderson, J.; Feiglin, M.; Sardana, M.; Griffin, P.; Schmatz, D.; Myers, R.W.
    Molecular cloning and functional expression of mannitol-1-phosphatase from the apicomplexan parasite Eimeria tenella (1998), J. Biol. Chem., 273, 4237-4244.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.22 medicine lack of mannitol metabolism in higher eukaryotes Eimeria tenella

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.22 cDNA from total RNA from unsporulated oocytes Eimeria tenella

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.22 diethyl dicarbonate at pH 6-8, due to histidine modification Eimeria tenella
3.1.3.22 NaCl inhibits native M1Pase with an IC50 value of 0.028 mM, for recombinant enzymes between 0.054 mM and 0.044 mM Eimeria tenella

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.22 cytosol
-
 Eimeria tenella 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.22 34650
-
 LC-ESI-MS analysis Eimeria tenella
3.1.3.22 34740
-
 calculation from sequence of cDNA Eimeria tenella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O Eimeria tenella mannitol metabolism D-mannitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.22 Eimeria tenella
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.22 side-chain modification by cDNA in comparison to the biochemical purified enzyme, active side H82 phosphorylated, detected by diethyl pyrocarbonate inhibition and reduced inhibition by p-nitrophenylphosphate Eimeria tenella

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.22 biochemical purification of native enzyme Eimeria tenella
3.1.3.22 recombinant fusion and His-tagged proteins Eimeria tenella

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.22 oocyte unsporulated Eimeria tenella
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O mannitol metabolism Eimeria tenella D-mannitol + phosphate
-
 ?
3.1.3.22 mannitol 1-phosphate + H2O
-
 Eimeria tenella mannitol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.22 monomer SDS-PAGE and western blot analysis Eimeria tenella

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.22 0.028
-
 inhibits native M1Pase with an IC50 value of 0.028 mM, for recombinant enzymes between 0.054 mM and 0.044 mM Eimeria tenella NaCl