EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.17 | 2-mercaptoethanol | maximal activation at 50 mM, absolute requirement for SH compounds | Oryctolagus cuniculus | |
3.1.3.17 | ascorbic acid | maximal activation at 1 mM | Oryctolagus cuniculus | |
3.1.3.17 | dithiothreitol | maximal activation at 1 mM, absolute requirement for SH compounds | Oryctolagus cuniculus |
EC Number | General Stability | Organism |
---|---|---|
3.1.3.17 | complete inactivation after treatment with 0.3 M perchloric acid or 10% trichloroacetic acid | Oryctolagus cuniculus |
3.1.3.17 | complete inactivation after treatment with 1% dodecyl sulfate or 0.05% cetyltrimethylammonium bromide or 1 M guanidine-HCl after 15 min | Oryctolagus cuniculus |
3.1.3.17 | complete inactivation after treatment with 10% w/w pronase at 30°C for 3 h | Oryctolagus cuniculus |
3.1.3.17 | resistant to trypsin and chymotrypsin and to 8 M urea | Oryctolagus cuniculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.17 | 2-hydroxyethyl disulfide | - |
Oryctolagus cuniculus | |
3.1.3.17 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Oryctolagus cuniculus | |
3.1.3.17 | Ca2+ | 10 mM, 20% inhibition | Oryctolagus cuniculus | |
3.1.3.17 | cystamine | - |
Oryctolagus cuniculus | |
3.1.3.17 | cystine | - |
Oryctolagus cuniculus | |
3.1.3.17 | D-Pantethine | - |
Oryctolagus cuniculus | |
3.1.3.17 | Disulfides | irreversible inactivation | Oryctolagus cuniculus | |
3.1.3.17 | GSSG | - |
Oryctolagus cuniculus | |
3.1.3.17 | iodoacetamide | 50 mM, 30% inhibition after 1 h at 30°C | Oryctolagus cuniculus | |
3.1.3.17 | iodoacetic acid | 50 mM, 30% inhibition after 1 h at 30°C | Oryctolagus cuniculus | |
3.1.3.17 | Mg2+ | 10 mM, 20% inhibition | Oryctolagus cuniculus | |
3.1.3.17 | Mn2+ | 3 mM, 64% inhibition | Oryctolagus cuniculus | |
3.1.3.17 | NaCl | 0.1-0.3 M, 40-96% inhibition | Oryctolagus cuniculus | |
3.1.3.17 | poly(L-lysine) | 0.001 mM, strong | Oryctolagus cuniculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.17 | Mn2+ | inactive in absence of Mn2+ | Oryctolagus cuniculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 33000 | - |
gel filtration | Oryctolagus cuniculus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.3.17 | deoxycholate | stable to 0.5% deoxycholate, 24 h | Oryctolagus cuniculus |
3.1.3.17 | Ethanol | stable to 25% ethanol at -5°C, 3 h | Oryctolagus cuniculus |
3.1.3.17 | Triton X-100 | stable to 0.5% Triton X-100, 24 h | Oryctolagus cuniculus |
3.1.3.17 | Tween | stable to 0.1% Tween 20, 40 or 80, 24 h | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.17 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.17 | no modification | the enzyme is free of bound phosphate, carbohydrate, lipids and nucleic acids | Oryctolagus cuniculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.17 | - |
Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.17 | muscle | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 1 | - |
- |
Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.17 | phosphorylase a + H2O | - |
Oryctolagus cuniculus | phosphorylase b + phosphate | - |
? | |
3.1.3.17 | troponin I + H2O | - |
Oryctolagus cuniculus | phosphate + ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 60 | - |
15 min, 50% loss of activity | Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 7.8 | - |
- |
Oryctolagus cuniculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 6.5 | 8.7 | about 50% of maximal activity at pH 6.5 and at pH 8.7 | Oryctolagus cuniculus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.17 | 5 | - |
30 min, 90% loss of activity | Oryctolagus cuniculus |