EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.1 | D369A | mutant enzyme shows reduced turnover rates and increased Km-value | Escherichia coli |
3.1.3.1 | D369N | mutant enzyme shows reduced turnover rates and increased Km-value. The reaction mechanism of the mutant enzyme involves only 1 metal with the possible assistance of a His side chain | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.1 | 0.0094 | - |
p-nitrophenyl phosphate | wild type enzyme, in absence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 0.02124 | - |
p-nitrophenyl phosphate | wild type enzyme, in presence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 0.05972 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in presence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 0.176 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in absence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 2.941 | - |
p-nitrophenyl phosphate | mutant enzyme D369A, in absence of a phosphate acceptor | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.1 | Escherichia coli | - |
- |
- |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.1 | 0.01 | - |
p-nitrophenyl phosphate | mutant enzyme D369A, in absence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 0.4 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in absence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 44.5 | - |
p-nitrophenyl phosphate | wild type enzyme | Escherichia coli | |
3.1.3.1 | 80.5 | - |
p-nitrophenyl phosphate | wild type enzyme, in presence of a phosphate acceptor | Escherichia coli | |
3.1.3.1 | 230 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in presence of a phosphate acceptor | Escherichia coli |