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Literature summary extracted from

  • Politino, M.; Brown, J.; Usher, J.J.
    Purification and characterization of an extracellular alkaline phosphatase from Penicillium chrysogenum (1996), Prep. Biochem. Biotechnol., 26, 171-181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Cu2+ weak inhibition Penicillium chrysogenum
3.1.3.1 EDTA
-
 Penicillium chrysogenum
3.1.3.1 Hg2+
-
 Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 1.3
-
 p-nitrophenyl phosphate
-
 Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 extracellular
-
 Penicillium chrysogenum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Mg2+ enhances activity to a lesser extent than Mg2+ Penicillium chrysogenum
3.1.3.1 Zn2+ enhances activity to a lesser extent than Mg2+ Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 58000
-
 1 * 58000, SDS-PAGE Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Penicillium chrysogenum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.1 side-chain modification contains 6-11% carbohydrate per weight Penicillium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 additional information
-
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 alpha-naphthyl phosphate + H2O
-
 Penicillium chrysogenum 1-naphthol + phosphate
-
 ?
3.1.3.1 etoposide phosphate + H2O
-
 Penicillium chrysogenum ?
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Penicillium chrysogenum p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 monomer 1 * 58000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 40 50
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 20 60 20°C: about 35% of maximal activity, 60°C: about 30% of maximal activity Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9
-
 hydrolysis of p-nitrophenyl phosphate Penicillium chrysogenum