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Literature summary extracted from

  • Say, J.C.; Ciuffi, K.; Furriel, R.P.M.; Ciancaglini, P.; Leone, F.A.
    Alkaline phosphatase from rat osseous plates: purification and biochemical characterization of a soluble form (1991), Biochim. Biophys. Acta, 1074, 256-262.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Zn2+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Ca2+ 20-25% stimulation Rattus norvegicus
3.1.3.1 Co2+ no stimulation Rattus norvegicus
3.1.3.1 Mg2+ 20-25% stimulation Rattus norvegicus
3.1.3.1 Mn2+ no stimulation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 80000
-
 2 * 80000, SDS-PAGE Rattus norvegicus
3.1.3.1 163000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 osseous plate
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 190.3
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 ATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.1 diphosphate + H2O
-
 Rattus norvegicus 2 phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 80000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 37
-
 very stable Rattus norvegicus
3.1.3.1 55
-
 15 min, about 80% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.5
-
 0.1 mM p-nitrophenyl phosphate Rattus norvegicus
3.1.3.1 10
-
 1 mM p-nitrophenyl phosphate Rattus norvegicus