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Literature summary extracted from

  • Dong, G.; Zeikus, J.G.
    Purification and characterization of alkaline phosphatase from Thermotoga neapolitana (1997), Enzyme Microb. Technol., 21, 335-340.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.183
-
 p-nitrophenyl phosphate
-
 Thermotoga neapolitana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Co2+ enhances activity, twice as active than with either Zn2+ or Mn2+ Thermotoga neapolitana
3.1.3.1 Mn2+ enhances activity Thermotoga neapolitana
3.1.3.1 Zn2+ enhances activity Thermotoga neapolitana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 45000
-
 2 * 45000, SDS-PAGE Thermotoga neapolitana
3.1.3.1 87000
-
 gel filtration Thermotoga neapolitana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Thermotoga neapolitana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Thermotoga neapolitana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 663.3
-
-
 Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 5'-ADP + H2O
-
 Thermotoga neapolitana ?
-
 ?
3.1.3.1 5'-ATP + H2O
-
 Thermotoga neapolitana ?
-
 ?
3.1.3.1 D-fructose 6-phosphate + H2O
-
 Thermotoga neapolitana D-fructose + phosphate
-
 ?
3.1.3.1 fructose 1,6-diphosphate + H2O
-
 Thermotoga neapolitana ?
-
 ?
3.1.3.1 glucose 1-phosphate + H2O
-
 Thermotoga neapolitana glucose + phosphate
-
 ?
3.1.3.1 Glucose 6-phosphate + H2O
-
 Thermotoga neapolitana Glucose + phosphate
-
 ?
3.1.3.1 glycerophosphate + H2O
-
 Thermotoga neapolitana glycerol + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Thermotoga neapolitana p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 45000, SDS-PAGE Thermotoga neapolitana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 85
-
-
 Thermotoga neapolitana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 60 95 60°C: about 45% of maximal activity, 9°C: about 60% of maximal activity Thermotoga neapolitana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 additional information
-
 thermostability is increased 30fold by Co2+ and 23fold by Mg2+ Thermotoga neapolitana
3.1.3.1 90
-
 half-life: 238 min, in presence of Co2+ Thermotoga neapolitana
3.1.3.1 100
-
 40 min, in presence of 40 mM Co2+, 3% loss of activity Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.9
-
-
 Thermotoga neapolitana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 8 11.5 pH 8.0: about 40% of maximal activity, pH 11.5: about 45% of maximal activity Thermotoga neapolitana