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Literature summary extracted from

  • Sakurai, Y.; Toda, K.; Shiota, H.
    Multiple forms and some properties of alkaline phosphatase produced by Aspergillus oryzae on solid medium (1981), Agric. Biol. Chem., 45, 1959-1967.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 arsenate 1 mM Na2HAsO4, 80% inhibition of isoenzyme I, 22% inhibition of isoenzyme II, 40% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 Ba2+ 1 mM, 95% inhibition of isoenzyme I Aspergillus oryzae
3.1.3.1 Borate 1 mM H3BO4, 16% inhibition of isoenzyme I, 13% inhibition of isoenzyme II, 8% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 Ca2+ weak inhibition of isoenzyme II Aspergillus oryzae
3.1.3.1 Cu2+ 1 mM, 87% inhibition of isoenzyme I, 10% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 EDTA inhibition of isoenzyme I and II Aspergillus oryzae
3.1.3.1 F- 1 mM NaF, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II Aspergillus oryzae
3.1.3.1 Hg2+ 1 mM nearly complete inhibition of isoenzyme I, II and III Aspergillus oryzae
3.1.3.1 Mg2+ weak inhibition of isoenzyme I Aspergillus oryzae
3.1.3.1 Mn2+ 1 mM 90% inhibition of isoenzyme I, 64% inhibition of isoenzyme II, 39% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 NaWO4 1 mM NaWO4, 35% inhibition of isoenzyme I, 80% inhibition of isoenzyme II, 14% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 PCMB 1 mM, 54% inhibition of isoenzyme I, 33% inhibition of isoenzyme II, 9% inhibition of isoenzyme III Aspergillus oryzae
3.1.3.1 phosphate 1 mM Na2HPO4, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II, 2% inhibition of isoenzyme III; competitive Aspergillus oryzae
3.1.3.1 Sr2+ 1 mM, 88% inhibition of isoenzyme I Aspergillus oryzae
3.1.3.1 Zn2+ 1 mM, 98% inhibition of isoenzyme I Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.11
-
 p-nitrophenyl phosphate isoenzyme III Aspergillus oryzae
3.1.3.1 0.22
-
 p-nitrophenyl phosphate isoenzyme II Aspergillus oryzae
3.1.3.1 2
-
 p-nitrophenyl phosphate isoenzyme I Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Ca2+ activates isoenzyme I, weak activation of isoenzyme III Aspergillus oryzae
3.1.3.1 Mg2+ activation of isoenzyme II and III Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Aspergillus oryzae
-
 three isoenzymes: I, II, III
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2',3'-CMP + H2O
-
 Aspergillus oryzae ?
-
 ?
3.1.3.1 2',3'-GMP + H2O
-
 Aspergillus oryzae ?
-
 ?
3.1.3.1 2',3-AMP + H2O
-
 Aspergillus oryzae ?
-
 ?
3.1.3.1 2-phosphoglycerate + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II Aspergillus oryzae glycerol + phosphate
-
 ?
3.1.3.1 3-phosphoglycerate + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I Aspergillus oryzae glycerol + phosphate
-
 ?
3.1.3.1 5'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.3.1 5'-dCMP + H2O
-
 Aspergillus oryzae ?
-
 ?
3.1.3.1 5'-GMP + H2O
-
 Aspergillus oryzae guanosine + phosphate
-
 ?
3.1.3.1 alpha-naphthyl phosphate + H2O hydrolyzed by isoenzyme II and III, no activity with isoenzyme I Aspergillus oryzae 1-naphthol + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O hydrolyzed by isoenzyme I, II or III Aspergillus oryzae p-nitrophenol + phosphate
-
 ?
3.1.3.1 phosphoethanolamine + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I Aspergillus oryzae phosphate + ethanolamine
-
 ?
3.1.3.1 phosphorylcholine + H2O hydrolyzed by isoenzyme III, no activity with isoenzyme I and II Aspergillus oryzae phosphate + choline
-
 ?
3.1.3.1 phosphoserine + H2O hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II Aspergillus oryzae phosphate + Ser
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 30
-
 pH 8, 120 min, stable Aspergillus oryzae
3.1.3.1 35
-
 pH 10, isoenzyme III, 120 min, stable Aspergillus oryzae
3.1.3.1 40
-
 pH 9-9.5, 120 min, stable Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8
-
 isoenzyme I Aspergillus oryzae
3.1.3.1 9 9.5 isoenzyme II Aspergillus oryzae
3.1.3.1 10
-
 isoenzyme III Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 7 10 30°C, stable Aspergillus oryzae