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Literature summary extracted from

  • Fernandez, M.P.; Gascon, S.; Schwenke, J.
    Some enzymatic properties of vacuolar alkaline phosphatase from yeast (1981), Curr. Microbiol., 6, 121-126.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 arsenate
-
 Cyberlindnera jadinii
3.1.3.1 Borate
-
 Cyberlindnera jadinii
3.1.3.1 EDTA
-
 Cyberlindnera jadinii
3.1.3.1 phosphate
-
 Cyberlindnera jadinii
3.1.3.1 Zn2+ 1 mM, 67% inhibition Cyberlindnera jadinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 2.5
-
 p-nitrophenyl phosphate
-
 Cyberlindnera jadinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 vacuole
-
 Cyberlindnera jadinii 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 135000
-
 gel filtration Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Cyberlindnera jadinii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 3'-ADP + H2O
-
 Cyberlindnera jadinii ?
-
 ?
3.1.3.1 5'-ADP + H2O
-
 Cyberlindnera jadinii ?
-
 ?
3.1.3.1 alpha-naphthyl phosphate + H2O
-
 Cyberlindnera jadinii 1-naphthol + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Cyberlindnera jadinii p-nitrophenol + phosphate
-
 ?
3.1.3.1 polyphosphate PP-25 + H2O
-
 Cyberlindnera jadinii ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 45
-
 pH 7.5, 60 min, rapid inactivation above Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8.9
-
-
 Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 7 10 pH 7.0: about 20% of maximal activity, pH 10.0: about 90% of maximal activity Cyberlindnera jadinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 7 9 5°C, 3 h, stable Cyberlindnera jadinii