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Literature summary extracted from

  • Korobi, H.; Sullivan, C.W.; Shizuya, H.
    Heat-labile alkaline phosphatase from antarctic bacteria: rapid 5' end-labeling of nucleic acids (1984), Proc. Natl. Acad. Sci. USA, 81, 6691-6695.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 67000
-
 gel filtration antarctic bacterium
3.1.3.1 68000
-
 1 * 68000, SDS-PAGE after treatment with 2-mercaptoethanol antarctic bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 antarctic bacterium
-
-
-
3.1.3.1 antarctic bacterium HK47
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 antarctic bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 90.3
-
-
 antarctic bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 ADP + H2O
-
 antarctic bacterium ?
-
 ?
3.1.3.1 ADP + H2O
-
 antarctic bacterium HK47 ?
-
 ?
3.1.3.1 AMP + H2O
-
 antarctic bacterium adenosine + phosphate
-
 ?
3.1.3.1 AMP + H2O
-
 antarctic bacterium HK47 adenosine + phosphate
-
 ?
3.1.3.1 GTP + H2O
-
 antarctic bacterium ?
-
 ?
3.1.3.1 GTP + H2O
-
 antarctic bacterium HK47 ?
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 antarctic bacterium p-nitrophenol + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 antarctic bacterium HK47 p-nitrophenol + phosphate
-
 ?
3.1.3.1 UTP + H2O
-
 antarctic bacterium ?
-
 ?
3.1.3.1 UTP + H2O
-
 antarctic bacterium HK47 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 monomer 1 * 68000, SDS-PAGE after treatment with 2-mercaptoethanol antarctic bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 25
-
-
 antarctic bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 10 40 10°C: about 30% of maximal activity, 40°C: about 10% of maximal activity antarctic bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 10
-
 10 min, little loss of activity antarctic bacterium
3.1.3.1 15
-
 10 min, 40% loss of activity antarctic bacterium
3.1.3.1 40
-
 half-life: 2 min antarctic bacterium
3.1.3.1 55
-
 10 min, complete and irreversible inactivation antarctic bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 4.5
-
 0°C, rapid and irreversible loss of activity at or below antarctic bacterium
3.1.3.1 7 9.5 0°C, stable antarctic bacterium