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Literature summary extracted from

  • von Tigerstrom R.G.; Stelmaschuk, S.
    Purification and characterization of the outer membrane associated alkaline phosphatase of Lysobacter enzymogenes (1986), J. Gen. Microbiol., 132, 1379-1387.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 8-hydroxyquinoline
-
 Lysobacter enzymogenes
3.1.3.1 EDTA
-
 Lysobacter enzymogenes
3.1.3.1 phosphate insensitive Lysobacter enzymogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.034
-
 p-nitrophenyl phosphate and a second Km value of 0.056 mM Lysobacter enzymogenes
3.1.3.1 0.056
-
 p-nitrophenyl phosphate and a second Km value of 0.034 mM Lysobacter enzymogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 membrane enzyme is associated with the outer membrane Lysobacter enzymogenes 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 69000
-
 2 * 69000, SDS-PAGE of the heat-denatured enzyme Lysobacter enzymogenes
3.1.3.1 76000
-
 2 * 76000, SDS-PAGE of the enzyme without heat treatment Lysobacter enzymogenes
3.1.3.1 150000
-
 gel filtration Lysobacter enzymogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Lysobacter enzymogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Lysobacter enzymogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 additional information
-
-
 Lysobacter enzymogenes

Storage Stability

EC Number Storage Stability Organism
3.1.3.1 -22°C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several weeks Lysobacter enzymogenes
3.1.3.1 0-4°C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several days Lysobacter enzymogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2',3'-GMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 2',3'-UMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 2'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
3.1.3.1 2-phosphoglycerate + H2O
-
 Lysobacter enzymogenes glycerol + phosphate
-
 ?
3.1.3.1 3'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
3.1.3.1 3-phosphoglycerate + H2O
-
 Lysobacter enzymogenes glycerol + phosphate
-
 ?
3.1.3.1 5'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
3.1.3.1 5'-CMP + H2O
-
 Lysobacter enzymogenes cytidine + phosphate
-
 ?
3.1.3.1 5'-dAMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 5'-dCMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 5'-dGMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 5'-dTMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 5'-GMP + H2O
-
 Lysobacter enzymogenes guanosine + phosphate
-
 ?
3.1.3.1 5'-UMP + H2O
-
 Lysobacter enzymogenes uridine + phosphate
-
 ?
3.1.3.1 ADP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 ATP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
3.1.3.1 Glucose 6-phosphate + H2O
-
 Lysobacter enzymogenes Glucose + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Lysobacter enzymogenes p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 69000, SDS-PAGE of the heat-denatured enzyme Lysobacter enzymogenes
3.1.3.1 dimer 2 * 76000, SDS-PAGE of the enzyme without heat treatment Lysobacter enzymogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 60
-
 stable for at least 20 min Lysobacter enzymogenes
3.1.3.1 70
-
 30% loss of activity after 10 min, 98% loss of activity after 20 min Lysobacter enzymogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8.5
-
-
 Lysobacter enzymogenes