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Literature summary extracted from

  • Eaton, R.H.; Moss, D.W.
    Partial purification and some properties of human bone alkaline phosphatase (1968), Enzymologia, 35, 31-39.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.1 urea, 3 M, 5 h, about 50% loss of activity of the liver enzyme, about 60% loss of activity of the bone enzyme. 5 M, 30 min, about 70% loss of activity of the liver enzyme, about 90% loss of activity of the bone enzyme. 6 M, 10 min, about 70% loss of activity of the liver enzyme, about 90% loss of activity of the bone enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Mg2+ strongly activates hydrolysis of p-nitrophenyl phosphate Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 200000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 bone
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 8
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 ADP + H2O
-
 Homo sapiens ?
-
 ?
3.1.3.1 AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.1 ATP + H2O
-
 Homo sapiens ?
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
3.1.3.1 thiamin diphosphate + H2O
-
 Homo sapiens thiamin phosphate + phosphate
-
 ?
3.1.3.1 UMP + H2O
-
 Homo sapiens uridine + phosphate
-
 ?
3.1.3.1 UTP + H2O
-
 Homo sapiens ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 54
-
 20 min, about 45% loss of activity of the liver enzyme, about 60% loss of activity of the bone enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9 9.5 hydrolysis of organic diphosphates Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 3.5
-
 about 50% loss of activity of the liver enzyme after 25 min, about 85% loss of activity of the bone enzyme after 5 min Homo sapiens
3.1.3.1 4.4
-
 about 65% loss of activity of the bone enzyme Homo sapiens
3.1.3.1 4.4
-
 160 min, about 40% loss of activity of the liver enzyme Homo sapiens