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Literature summary extracted from

  • Spencer, T.; Macrae, S.L.
    Some properties of the alkaline phosphatase of HeLa cells (1972), Enzymologia, 42, 329-341.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Be2+
-
 Homo sapiens
3.1.3.1 Phe
-
 Homo sapiens
3.1.3.1 Zn2+ above 0.02 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 cytoplasm
-
 Homo sapiens 5737
-
3.1.3.1 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Mg2+ activates Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 64
-
 120 min, about 15% loss of activity of the cytolasmic isoenzyme, about 5% loss of activity of the nuclear isoenzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.2
-
 hydrolysis of 2 mM p-nitrophenyl phosphate, borate buffer Homo sapiens
3.1.3.1 9.8
-
 hydrolysis of 2 mM p-nitrophenyl phosphate, 2-amino-2-methylpropan-1-ol hydrochloride buffer Homo sapiens
3.1.3.1 10.5
-
 hydrolysis of 8 mM p-nitrophenyl phosphate, 2-amino-2-methylpropan-1-ol hydrochloride buffer Homo sapiens