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Literature summary extracted from

  • Heimo, H.; Palmu, K.; Suominen, I.
    Human placental alkaline phosphatase: expression in Pichia pastoris, purification and characterization (1998), Protein Expr. Purif., 12, 85-92.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 expression in Pichia pastoris Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.94
-
 p-nitrophenyl phosphate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 soluble
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 62000
-
 x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE Homo sapiens
3.1.3.1 65000
-
 x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.1 side-chain modification the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation. There are two putative N-glycosylation sites in the primary sequence, Asn122-Thr-Thr and Asn249-Arg-Thr, only the latter appears to be glycosylated in the naive enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE Homo sapiens