EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.4 | acetoacetyl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | acetyl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | DL-3-hydroxybutyryl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | DL-methylmalonyl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | isobutyryl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | malonyl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | n-valeryl-CoA | competitive | Rattus norvegicus | |
3.1.2.4 | propionyl-CoA | competitive | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.4 | 0.006 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus | |
3.1.2.4 | 0.025 | - |
3-hydroxypropionyl-CoA | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.2.4 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.4 | 36000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.4 | 3-hydroxy-2-methylpropanoyl-CoA + H2O | Rattus norvegicus | enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.4 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.4 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.2.4 | brain | - |
Rattus norvegicus | - |
3.1.2.4 | heart | - |
Rattus norvegicus | - |
3.1.2.4 | kidney | - |
Rattus norvegicus | - |
3.1.2.4 | liver | - |
Rattus norvegicus | - |
3.1.2.4 | muscle | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.2.4 | 427 | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.4 | (S)-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | CoA + methylmalonate | - |
? | |
3.1.2.4 | 3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3.1.2.4 | 3-hydroxy-2-methylpropanoyl-CoA + H2O | enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus | ? | - |
? | |
3.1.2.4 | 3-hydroxypropanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + 3-hydroxypropanoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.4 | monomer | 1 * 36000, SDS-PAGE | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.4 | 150 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus | |
3.1.2.4 | 266 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.4 | 6 | - |
hydrolysis of (S)-methylmalonyl-CoA | Rattus norvegicus |
3.1.2.4 | 8 | - |
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.4 | 5 | 7 | pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA | Rattus norvegicus |
3.1.2.4 | 5 | 10.5 | pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus |