BRENDA - Enzyme Database

Purification and partial characterization of 3-hydroxyisobutyryl-coenzyme A hydrolase of rat liver

Shimomura, Y.; Murakami, T.; Fujitsuka, N.; Nakai, N.; Sato, Y.; Sugiyama, S.; Shimomura, N.; Irwin, J.; Hawes, J.W.; Harris, R.A.; J. Biol. Chem. 269, 14248-14253 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.4
acetoacetyl-CoA
competitive
Rattus norvegicus
3.1.2.4
acetyl-CoA
competitive
Rattus norvegicus
3.1.2.4
DL-3-hydroxybutyryl-CoA
competitive
Rattus norvegicus
3.1.2.4
DL-methylmalonyl-CoA
competitive
Rattus norvegicus
3.1.2.4
isobutyryl-CoA
competitive
Rattus norvegicus
3.1.2.4
malonyl-CoA
competitive
Rattus norvegicus
3.1.2.4
n-valeryl-CoA
competitive
Rattus norvegicus
3.1.2.4
propionyl-CoA
competitive
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.4
0.006
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
3.1.2.4
0.025
-
3-hydroxypropionyl-CoA
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.4
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.4
36000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
Rattus norvegicus
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.2.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.2.4
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.4
brain
-
Rattus norvegicus
-
3.1.2.4
heart
-
Rattus norvegicus
-
3.1.2.4
kidney
-
Rattus norvegicus
-
3.1.2.4
liver
-
Rattus norvegicus
-
3.1.2.4
muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.4
427
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.2.4
(S)-methylmalonyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + methylmalonate
-
-
-
?
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxy-2-methylpropanoate
-
-
-
?
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
94558
Rattus norvegicus
?
-
-
-
?
3.1.2.4
3-hydroxypropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxypropanoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.4
monomer
1 * 36000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.4
150
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
3.1.2.4
266
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.4
6
-
hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
3.1.2.4
8
-
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.4
5
7
pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
3.1.2.4
5
10.5
pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.4
acetoacetyl-CoA
competitive
Rattus norvegicus
3.1.2.4
acetyl-CoA
competitive
Rattus norvegicus
3.1.2.4
DL-3-hydroxybutyryl-CoA
competitive
Rattus norvegicus
3.1.2.4
DL-methylmalonyl-CoA
competitive
Rattus norvegicus
3.1.2.4
isobutyryl-CoA
competitive
Rattus norvegicus
3.1.2.4
malonyl-CoA
competitive
Rattus norvegicus
3.1.2.4
n-valeryl-CoA
competitive
Rattus norvegicus
3.1.2.4
propionyl-CoA
competitive
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.4
0.006
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
3.1.2.4
0.025
-
3-hydroxypropionyl-CoA
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.4
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.4
36000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
Rattus norvegicus
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.4
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.4
brain
-
Rattus norvegicus
-
3.1.2.4
heart
-
Rattus norvegicus
-
3.1.2.4
kidney
-
Rattus norvegicus
-
3.1.2.4
liver
-
Rattus norvegicus
-
3.1.2.4
muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.4
427
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.2.4
(S)-methylmalonyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + methylmalonate
-
-
-
?
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxy-2-methylpropanoate
-
-
-
?
3.1.2.4
3-hydroxy-2-methylpropanoyl-CoA + H2O
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
94558
Rattus norvegicus
?
-
-
-
?
3.1.2.4
3-hydroxypropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxypropanoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.4
monomer
1 * 36000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.4
150
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
3.1.2.4
266
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.4
6
-
hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
3.1.2.4
8
-
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.4
5
7
pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
3.1.2.4
5
10.5
pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus