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Literature summary extracted from

  • Kovacs, K.; Szajani, B.; Boross, L.
    Preparation and properties of a novel immobilized cholinesterase (1982), J. Appl. Biochem., 4, 11-18.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.8 analysis enzyme in immobilized forms has a potential application in environmental, water and air, pollution control as a functional part of monitoring system Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.33
-
 butyrylthiocholine immobilized enzyme Equus caballus
3.1.1.8 1.87
-
 butyrylthiocholine soluble enzyme Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Equus caballus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 serum
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 additional information
-
-
 Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetylcholine + H2O acetylcholine iodide Equus caballus acetate + choline
-
 ?
3.1.1.8 acetylthiocholine + H2O acetylthiocholine iodide Equus caballus acetate + thiocholine
-
 ?
3.1.1.8 butyrylcholine + H2O butyrylcholine iodide Equus caballus butanoate + choline
-
 ?
3.1.1.8 butyrylthiocholine + H2O butyrylthiocholine iodide Equus caballus butanoate + thiocholine
-
 ?
3.1.1.8 succinylcholine + H2O succinylcholine chloride Equus caballus succinate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 acylcholine acyl-hydrolase
-
 Equus caballus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.8 34
-
 immobilized enzyme Equus caballus
3.1.1.8 36 37 soluble enzyme Equus caballus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.8 50
-
 60 min, 40% loss of activity, immobilized enzyme Equus caballus
3.1.1.8 60
-
 60 min, complete loss of activity, immobilized enzyme Equus caballus
3.1.1.8 62
-
 30 min, complete loss of activity, immobilized enzyme Equus caballus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7.6
-
 25°C, soluble enzyme Equus caballus
3.1.1.8 8
-
 immobilized enzyme Equus caballus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.8 5.5 8.5 soluble and immobilized enzyme Equus caballus