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Literature summary extracted from

  • Saeed, A.; De Boeck, St.; Debruyne, I.; Wouters, J.; Stockx, J.
    Hen's egg yolk cholinesterase (1980), Biochim. Biophys. Acta, 614, 389-399.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.8 citrate 20 mM, 140% activity Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 N,N,N',N'-tetramethyl-1,6-hexane diamine
-
 Gallus gallus
3.1.1.8 NaCl
-
 Gallus gallus
3.1.1.8 physostigmine i.e. eserine Gallus gallus
3.1.1.8 tetrabutylammonium competitive Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.8 Ba2+ activates Gallus gallus
3.1.1.8 Mg2+ 20 mM activates Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.8 104000
-
 yolk and liver cholinesterase 2, gel filtration Gallus gallus
3.1.1.8 440000
-
 yolk and liver cholinesterase 1, blood plasma cholinesterase, gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Anas platyrhynchos
-
-
-
3.1.1.8 Anser sp.
-
-
-
3.1.1.8 Gallus gallus
-
 hen
-
3.1.1.8 Pavo sp.
-
-
-
3.1.1.8 pheasant
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8 partial, 2 yolk, 2 liver and 1 blood plasma enzyme Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 egg
-
 Anas platyrhynchos
-
3.1.1.8 egg
-
 Anser sp.
-
3.1.1.8 egg
-
 Pavo sp.
-
3.1.1.8 egg
-
 pheasant
-
3.1.1.8 egg egg yolk Gallus gallus
-
3.1.1.8 liver laying hens Gallus gallus
-
3.1.1.8 plasma
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
3.1.1.8 -16°C, enzyme retains full activity as lyophilized powder after 6 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetyl-beta-methylcholine + H2O poor substrate Gallus gallus acetate + beta-methylcholine
-
 ?
3.1.1.8 acetyl-beta-methylcholine + H2O acetyl-beta-methylcholine chloride Gallus gallus acetate + beta-methylcholine
-
 ?
3.1.1.8 acetylcholine + H2O acetylcholine chloride Gallus gallus acetate + choline
-
 ?
3.1.1.8 acetylthiocholine + H2O acetylthiocholine iodide Gallus gallus acetate + thiocholine
-
 ?
3.1.1.8 benzoylcholine + H2O benzoylcholine chloride Gallus gallus benzoate + choline
-
 ?
3.1.1.8 butyrylcholine + H2O butyrylcholine chloride Gallus gallus butanoate + choline
-
 ?
3.1.1.8 butyrylthiocholine + H2O butyrylthiocholine iodide Gallus gallus butanoate + thiocholine
-
 ?
3.1.1.8 propionylcholine + H2O propionylcholine chloride Gallus gallus propanoate + choline
-
 ?
3.1.1.8 propionylthiocholine + H2O propionylthiocholine iodide Gallus gallus propanoate + thiocholine
-
 ?
3.1.1.8 succinyl dicholine dichloride + H2O yolk cholinesterase 1 and 2 and blood plasma cholinesterase poor substrate Gallus gallus ?
-
 ?
3.1.1.8 succinyl dicholine dichloride + H2O liver cholinesterase 1 and 2 no substrate Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 acylcholine acyl-hydrolase
-
 Gallus gallus
3.1.1.8 acylcholine acyl-hydrolase
-
 Anas platyrhynchos
3.1.1.8 acylcholine acyl-hydrolase
-
 Anser sp.
3.1.1.8 acylcholine acyl-hydrolase
-
 Pavo sp.
3.1.1.8 acylcholine acyl-hydrolase
-
 pheasant

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.8 70
-
 10 min, pH 7.2, complete loss of activity Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.8 6 9 25°C, excellent stability Gallus gallus