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Literature summary extracted from

  • Riov, J.; Jaffe, M.J.
    Cholinesterases from plant tissues (1973), Plant Physiol., 51, 520-528.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 (2-mercaptoethyl)trimethylammonium iodide O,O-diethyl phosphorothioate 0.2 mM, 21% inhibition; i.e. echothiophate Vigna radiata var. radiata
3.1.1.8 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one 1 mM, 20% inhibition, 10 mM, 65% inhibition; i.e. B.W 284 C51 dibromide Vigna radiata var. radiata
3.1.1.8 1-methylcarbamoyloxy-naphthalene 0.2 mM, 19% inhibition; i.e. sevin Vigna radiata var. radiata
3.1.1.8 1-p-menthanol, 2-dimethylamino-, 2,5-dimethylbenzyl chloride Q 80, growth retardant, 0.1 mM, 32% inhibition Vigna radiata var. radiata
3.1.1.8 ambenonium 1 mM, 18% inhibition, 10 mM 50% inhibition Vigna radiata var. radiata
3.1.1.8 ammonium(5-hydroxycarvacryl) trimethyl chloride piperidine carboxylate i.e. AMO-1618, growth retardant, 1 mM, 85% inhibition Vigna radiata var. radiata
3.1.1.8 Diethyl p-nitrophenyl phosphate 0.001 mM, 71% inhibition; i.e. paraoxon Vigna radiata var. radiata
3.1.1.8 ethopropazine hydrochloride 1 mM, 28% inhibition Vigna radiata var. radiata
3.1.1.8 neostigmine 0.0006 mM, 50% inhibition Vigna radiata var. radiata
3.1.1.8 physostigmine 0.42 mM, 50% inhibition; i.e. eserine Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.072
-
 Acetylcholine
-
 Vigna radiata var. radiata
3.1.1.8 0.084
-
 acetylthiocholine
-
 Vigna radiata var. radiata

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 root
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 0.096
-
 acetylcholine Vigna radiata var. radiata

Storage Stability

EC Number Storage Stability Organism
3.1.1.8 4°C, several weeks, no loss of activity, enzyme preparation obtained by direct extraction with (NH4)2SO4 Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetylcholine + H2O
-
 Vigna radiata var. radiata acetate + choline
-
 ?
3.1.1.8 acetylthiocholine + H2O
-
 Vigna radiata var. radiata acetate + thiocholine
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Vigna radiata var. radiata butanoate + thiocholine
-
 ?
3.1.1.8 additional information
-
 Vigna radiata var. radiata ?
-
 ?
3.1.1.8 propionylthiocholine + H2O
-
 Vigna radiata var. radiata propanoate + thiocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 ChE
-
 Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 8.5 8.7 acetylcholine Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.8 6 10 pH 6.0 and pH 10.0 25% of maximal activity Vigna radiata var. radiata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.8 0.0006
-
 neostigmine
-
 Vigna radiata var. radiata
3.1.1.8 10
-
 ambenonium
-
 Vigna radiata var. radiata