Characterization of wax-ester hydrolase from roots of white mustard (Sinapis alba L.) seedlings
Kalinowska, M.; Wojciechowski, Z.A.; Acta Biochim. Pol. 32, 263-269 (1985)
No PubMed abstract available
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.50
deoxycholate
-
Sinapis alba
3.1.1.50
DTT
5 mM, 40% stimulation
Sinapis alba
3.1.1.50
Triton X-100
stimulates
Sinapis alba
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.50
0.0045
-
cholesteryl palmitate
-
Sinapis alba
3.1.1.50
0.028
-
hexadecanyl palmitate
-
Sinapis alba
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.50
membrane
-
Sinapis alba
16020
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.50
Sinapis alba
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.50
-
Sinapis alba
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.50
root
seedlings
Sinapis alba
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.50
0.074
-
-
Sinapis alba
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.50
butyl palmitate + H2O
76% of the activity with cholesteryl palmitate
94402
Sinapis alba
butanol + palmitate
-
-
-
?
3.1.1.50
cholesteryl palmitate + H2O
-
94402
Sinapis alba
cholesterol + palmitate
-
-
-
?
3.1.1.50
decanyl palmitate + H2O
84% of the activity with cholesteryl palmitate
94402
Sinapis alba
decanol + palmitate
-
-
-
?
3.1.1.50
docosanyl palmitate + H2O
69% of the activity with cholesteryl palmitate
94402
Sinapis alba
docosanol + palmitate
-
-
-
?
3.1.1.50
dodecanyl palmitate + H2O
85% of the activity with cholesteryl palmitate
94402
Sinapis alba
dodecanol + palmitate
-
-
-
?
3.1.1.50
eicosanyl palmitate + H2O
72% of the activity with cholesteryl palmitate
94402
Sinapis alba
eicosanol + palmitate
-
-
-
?
3.1.1.50
ethyl palmitate + H2O
69% of the activity with cholesteryl palmitate
94402
Sinapis alba
ethanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl acetate + H2O
3% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + acetate
-
-
-
?
3.1.1.50
hexadecanyl arachidate + H2O
27% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + arachidate
-
-
-
?
3.1.1.50
hexadecanyl behenic acid ester + H2O
21% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + behenic acid
-
-
-
?
3.1.1.50
hexadecanyl butyrate + H2O
10% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + butyrate
-
-
-
?
3.1.1.50
hexadecanyl caprylate + H2O
25% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + caprylate
-
-
-
?
3.1.1.50
hexadecanyl laurate + H2O
32% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + laurate
-
-
-
?
3.1.1.50
hexadecanyl myristate + H2O
36% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + myristate
-
-
-
?
3.1.1.50
hexadecanyl palmitate + H2O
-
94402
Sinapis alba
hexadecanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl palmitate + H2O
77% of the activity with cholesteryl palmitate
94402
Sinapis alba
hexadecanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl stearate + H2O
48% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + stearate
-
-
-
?
3.1.1.50
octadecanyl palmitate + H2O
72% of the activity with cholesteryl palmitate
94402
Sinapis alba
octadecanol + palmitate
-
-
-
?
3.1.1.50
octanyl palmitate + H2O
92% of the activity with cholesteryl palmitate
94402
Sinapis alba
octanol + palmitate
-
-
-
?
3.1.1.50
tetraeicosanyl palmitate + H2O
61% of the activity with cholesteryl palmitate
94402
Sinapis alba
tetraeicosanol + palmitate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.50
5.8
8.5
broad optimum without definite maximum peak
Sinapis alba
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.50
5.5
9
activity sharply decreases below pH 5.5. and above pH 9.0
Sinapis alba
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.50
deoxycholate
-
Sinapis alba
3.1.1.50
DTT
5 mM, 40% stimulation
Sinapis alba
3.1.1.50
Triton X-100
stimulates
Sinapis alba
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.50
0.0045
-
cholesteryl palmitate
-
Sinapis alba
3.1.1.50
0.028
-
hexadecanyl palmitate
-
Sinapis alba
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.50
membrane
-
Sinapis alba
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.50
-
Sinapis alba
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.50
root
seedlings
Sinapis alba
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.50
0.074
-
-
Sinapis alba
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.50
butyl palmitate + H2O
76% of the activity with cholesteryl palmitate
94402
Sinapis alba
butanol + palmitate
-
-
-
?
3.1.1.50
cholesteryl palmitate + H2O
-
94402
Sinapis alba
cholesterol + palmitate
-
-
-
?
3.1.1.50
decanyl palmitate + H2O
84% of the activity with cholesteryl palmitate
94402
Sinapis alba
decanol + palmitate
-
-
-
?
3.1.1.50
docosanyl palmitate + H2O
69% of the activity with cholesteryl palmitate
94402
Sinapis alba
docosanol + palmitate
-
-
-
?
3.1.1.50
dodecanyl palmitate + H2O
85% of the activity with cholesteryl palmitate
94402
Sinapis alba
dodecanol + palmitate
-
-
-
?
3.1.1.50
eicosanyl palmitate + H2O
72% of the activity with cholesteryl palmitate
94402
Sinapis alba
eicosanol + palmitate
-
-
-
?
3.1.1.50
ethyl palmitate + H2O
69% of the activity with cholesteryl palmitate
94402
Sinapis alba
ethanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl acetate + H2O
3% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + acetate
-
-
-
?
3.1.1.50
hexadecanyl arachidate + H2O
27% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + arachidate
-
-
-
?
3.1.1.50
hexadecanyl behenic acid ester + H2O
21% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + behenic acid
-
-
-
?
3.1.1.50
hexadecanyl butyrate + H2O
10% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + butyrate
-
-
-
?
3.1.1.50
hexadecanyl caprylate + H2O
25% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + caprylate
-
-
-
?
3.1.1.50
hexadecanyl laurate + H2O
32% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + laurate
-
-
-
?
3.1.1.50
hexadecanyl myristate + H2O
36% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + myristate
-
-
-
?
3.1.1.50
hexadecanyl palmitate + H2O
-
94402
Sinapis alba
hexadecanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl palmitate + H2O
77% of the activity with cholesteryl palmitate
94402
Sinapis alba
hexadecanol + palmitate
-
-
-
?
3.1.1.50
hexadecanyl stearate + H2O
48% of the activity with hexadecanyl palmitate
94402
Sinapis alba
hexadecanol + stearate
-
-
-
?
3.1.1.50
octadecanyl palmitate + H2O
72% of the activity with cholesteryl palmitate
94402
Sinapis alba
octadecanol + palmitate
-
-
-
?
3.1.1.50
octanyl palmitate + H2O
92% of the activity with cholesteryl palmitate
94402
Sinapis alba
octanol + palmitate
-
-
-
?
3.1.1.50
tetraeicosanyl palmitate + H2O
61% of the activity with cholesteryl palmitate
94402
Sinapis alba
tetraeicosanol + palmitate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.50
5.8
8.5
broad optimum without definite maximum peak
Sinapis alba
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.50
5.5
9
activity sharply decreases below pH 5.5. and above pH 9.0
Sinapis alba