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Literature summary extracted from

  • Zhang, Y.; Dennis, E.A.
    Purification and characterization of a lysophospholipase from a macrophage-like cell line P388D1 (1988), J. Biol. Chem., 263, 9965-9972.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.5 -20°C, in absence of glycerol, the enzyme loses all of its activity during the freezing and thawing process Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide
-
 Mus musculus
3.1.1.5 Ca2+
-
 Mus musculus
3.1.1.5 Co2+
-
 Mus musculus
3.1.1.5 Cu2+
-
 Mus musculus
3.1.1.5 Fe2+
-
 Mus musculus
3.1.1.5 Fe3+ partial Mus musculus
3.1.1.5 Hg2+
-
 Mus musculus
3.1.1.5 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.5 0.022
-
 lysophosphatidylcholine substrate concentration between 0.015-0.2 mM Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 27000
-
 x * 27000, lysophospholipase I, SDS-PAGE Mus musculus
3.1.1.5 28000
-
 x * 28000, lysophospholipase II, SDS-PAGE Mus musculus
3.1.1.5 30000
-
 lysophospholipase I, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Mus musculus
-
 lysophospholipase I and II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.5 P-388D1 cell macrophage-like Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.5 1.34
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.1.1.5 -20°C, enzyme concentration: 0.01-0.025 mg/ml, 60-70% glycerol, stable for several months Mus musculus
3.1.1.5 4°C, stable for several weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 lysophosphatidylcholine + H2O
-
 Mus musculus glycerophosphorylcholine + unesterified fatty acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 28000, lysophospholipase II, SDS-PAGE Mus musculus
3.1.1.5 ? x * 27000, lysophospholipase I, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 7.5 9
-
 Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.5 6 8.5 unstable below pH 6.0 and above pH 8.5 Mus musculus