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Literature summary extracted from

  • Witt, W.; Schweingruber, M.E.; Mertsching, A.
    Phospholipase B from the plasma membrane of Saccharomyces cerevisiae (1984), Biochim. Biophys. Acta, 795, 108-116.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 Ca2+ CaCl2, reversed by EDTA Saccharomyces cerevisiae
3.1.1.5 Mg2+ MgCl2, reversed by EDTA Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.5 membrane
-
 Saccharomyces cerevisiae 16020
-
3.1.1.5 plasma membrane
-
 Saccharomyces cerevisiae 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 145000
-
 x * 145000, phospholipase B2, SDS-PAGE Saccharomyces cerevisiae
3.1.1.5 220000
-
 x * 220000, phospholipase B1, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Saccharomyces cerevisiae
-
 2 enzyme forms: phospholipase B1 and phospholipase B2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.5 side-chain modification glycoprotein Saccharomyces cerevisiae
3.1.1.5 side-chain modification phospholipase B2 contains 0.0104 mM carbohydrate per mg protein Saccharomyces cerevisiae
3.1.1.5 side-chain modification phospholipase B1 contains 0.0207 mM mannose per mg of protein Saccharomyces cerevisiae
3.1.1.5 side-chain modification phospholipase 2 contains 0.0097 mM mannose per mg of protein Saccharomyces cerevisiae
3.1.1.5 side-chain modification phospholipase B1 contains 0.0224 mM carbohydrate per mg protein Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 lysophosphatidylcholine + H2O
-
 Saccharomyces cerevisiae glycerophosphorylcholine + unesterified fatty acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 220000, phospholipase B1, SDS-PAGE Saccharomyces cerevisiae
3.1.1.5 ? x * 145000, phospholipase B2, SDS-PAGE Saccharomyces cerevisiae