BRENDA - Enzyme Database

Purification and some properties of phospholipase B from Schizosaccharomyces pombe

Oishi, H.; Tsuda, S.; Watanabe, Y.; Tamai, Y.; Biosci. Biotechnol. Biochem. 60, 1087-1092 (1996)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
sodium deoxycholate
0.1%, stimulates
Schizosaccharomyces pombe
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
cetyltrimethylammonium bromide
-
Schizosaccharomyces pombe
3.1.1.5
EDTA
-
Schizosaccharomyces pombe
3.1.1.5
Fe2+
-
Schizosaccharomyces pombe
3.1.1.5
Fe3+
-
Schizosaccharomyces pombe
3.1.1.5
SDS
-
Schizosaccharomyces pombe
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Ca2+
CaCl2, 10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
CoCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
CuSO4
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
HgCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
MgCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
MnCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
NiSO4
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
ZnCl2
10 mM, stimulates
Schizosaccharomyces pombe
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
300000
-
gel filtration
Schizosaccharomyces pombe
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.5
Schizosaccharomyces pombe
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
side-chain modification
glycoprotein
Schizosaccharomyces pombe
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.5
-
Schizosaccharomyces pombe
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
2.71
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
lysophosphatidylcholine + H2O
249% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
glycerophosphorylcholine + unesterified fatty acid
-
94366
Schizosaccharomyces pombe
?
3.1.1.5
lysophosphatidylethanolamine + H2O
27% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
fatty acid + glycerylphosphoethanolamine
-
-
-
?
3.1.1.5
phosphatidic acid + H2O
62% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
?
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
94366
Schizosaccharomyces pombe
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
819% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
22% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.5
dimer
2 * or 3 x * 100000-150000, SDS-PAGE
Schizosaccharomyces pombe
3.1.1.5
trimer
2 * or 3 x * 100000-150000, SDS-PAGE
Schizosaccharomyces pombe
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.5
35
-
-
Schizosaccharomyces pombe
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
55
-
30 min, 95% loss of activity
Schizosaccharomyces pombe
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
2.5
-
-
Schizosaccharomyces pombe
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.5
2.2
3
pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity
Schizosaccharomyces pombe
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.1.5
2.2
6.5
stable between pH 2.2 and pH 6.5
Schizosaccharomyces pombe
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
sodium deoxycholate
0.1%, stimulates
Schizosaccharomyces pombe
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
cetyltrimethylammonium bromide
-
Schizosaccharomyces pombe
3.1.1.5
EDTA
-
Schizosaccharomyces pombe
3.1.1.5
Fe2+
-
Schizosaccharomyces pombe
3.1.1.5
Fe3+
-
Schizosaccharomyces pombe
3.1.1.5
SDS
-
Schizosaccharomyces pombe
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Ca2+
CaCl2, 10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
CoCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
CuSO4
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
HgCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
MgCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
MnCl2
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
NiSO4
10 mM, stimulates
Schizosaccharomyces pombe
3.1.1.5
ZnCl2
10 mM, stimulates
Schizosaccharomyces pombe
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
300000
-
gel filtration
Schizosaccharomyces pombe
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
side-chain modification
glycoprotein
Schizosaccharomyces pombe
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
-
Schizosaccharomyces pombe
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
2.71
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
lysophosphatidylcholine + H2O
249% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
glycerophosphorylcholine + unesterified fatty acid
-
94366
Schizosaccharomyces pombe
?
3.1.1.5
lysophosphatidylethanolamine + H2O
27% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
fatty acid + glycerylphosphoethanolamine
-
-
-
?
3.1.1.5
phosphatidic acid + H2O
62% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
?
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
94366
Schizosaccharomyces pombe
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
819% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
22% of the activity with phosphatidylcholine
94366
Schizosaccharomyces pombe
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.5
dimer
2 * or 3 x * 100000-150000, SDS-PAGE
Schizosaccharomyces pombe
3.1.1.5
trimer
2 * or 3 x * 100000-150000, SDS-PAGE
Schizosaccharomyces pombe
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.5
35
-
-
Schizosaccharomyces pombe
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
55
-
30 min, 95% loss of activity
Schizosaccharomyces pombe
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
2.5
-
-
Schizosaccharomyces pombe
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.5
2.2
3
pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity
Schizosaccharomyces pombe
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.1.5
2.2
6.5
stable between pH 2.2 and pH 6.5
Schizosaccharomyces pombe