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Literature summary extracted from

  • Pathak, D.; Ashley, G.; Ollis, D.
    Thiol protease-like active site found in the enzyme dienelactone hydrolase: localization using biochemical, genetic, and structural tools (1991), Proteins Struct. Funct. Genet., 9, 267-279.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.45 refined crystal structure of DLH at 1.8 A, C123S DLH at 2.2 A, C123A at 2.0 A resolution Pseudomonas knackmussii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.45 C123A inactive Pseudomonas knackmussii
3.1.1.45 C123S burst kinetics with p-nitrophenyl acetate, 10% as active as DLH Pseudomonas knackmussii
3.1.1.45 C60S no reduction in activity Pseudomonas knackmussii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.17
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
3.1.1.45 0.22
-
 trans-4-Carboxymethylenebut-2-en-4-olide C60S DLH Pseudomonas knackmussii
3.1.1.45 9.9
-
 trans-4-Carboxymethylenebut-2-en-4-olide C123S DLH Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas knackmussii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii
3.1.1.45 C123A DLH Pseudomonas knackmussii
3.1.1.45 C123S DLH Pseudomonas knackmussii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate mechanism Pseudomonas knackmussii
a
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate esterase activity on chromophoric ester substrate, p-nitrophenyl acetate and the synthetic active-site titrant, trans-cinnamoyl imidazole Pseudomonas knackmussii
a
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases Pseudomonas knackmussii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 p-nitrophenyl acetate + H2O esterase activity Pseudomonas knackmussii p-nitrophenol + acetate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-cinnamoyl imidazole + H2O esterase activity Pseudomonas knackmussii trans-cinnamate + imidazole
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer alpha,beta protein Pseudomonas knackmussii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.45 DLH
-
 Pseudomonas knackmussii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 2.67
-
 trans-4-Carboxymethylenebut-2-en-4-olide C123S DLH Pseudomonas knackmussii
3.1.1.45 9.17
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
3.1.1.45 15
-
 trans-4-Carboxymethylenebut-2-en-4-olide C60S DLH Pseudomonas knackmussii